Production, characterisation and applicability of monoclonal antibodies to European eel ( Anguilla anguilla L., 1758) immunoglobulin

• Published in: Veterinary immunology and immunopathology 1995, Vol.45 (1), p.151-164
• Main Authors: van der Heijden, M.H.T., Rooijakkers, J.B.M.A., Booms, G.H.R., Rombout, J.H.W.M., Boon, J.H.
• Format: Article
• Language: English
• Subjects: Anguilla - immunology; Anguilla anguilla; Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity - immunology; Aquaculture and Fisheries; Aquacultuur en Visserij; B-Lymphocytes - immunology; B-Lymphocytes - ultrastructure; Blotting, Western - veterinary; Chromatography, Affinity - veterinary; Enzyme-Linked Immunosorbent Assay - veterinary; Experimental Animal Morphology and Cell Biology; Experimentele diermorfologie en celbiologie; Female; Fluorescent Antibody Technique - veterinary; Freshwater; Immunoglobulin G - immunology; Immunoglobulin G - isolation & purification; Immunoglobulin Heavy Chains - immunology; Immunoglobulin Light Chains - immunology; Leerstoelgroep Aquacultuur en Visserij; Male; Mice; Mice, Inbred BALB C; Microscopy, Immunoelectron - veterinary; WIAS
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/0165-2427(94)05335-P

Monoclonal antibodies (mAbs) to European eel ( Anguilla anguilla L., 1758) immunoglobulin (Ig) were produced, characterised and tested for applicability in a number of immuno(cyto)chemical assays. The selected mAbs, WEI 1 and WEI 2, were specifically reactive with Ig heavy and light chain, respectively. WEI 1 appeared to react with all or nearly all Ig molecules, B cells and plasma cells. WEI 2 was reactive with a subpopulation of those cells, indicating that European eel possesses at least two antigenically different light chain types. Both mAbs could be used for detection of antigen-specific antibodies in plasma by means of an enzyme-linked immunosorbent assay (ELISA).