Influence of flumequine on in vivo mitogen responses of European eel ( Anguilla anguilla L., 1758) lymphoid cells

• Published in: Veterinary immunology and immunopathology 1995-07, Vol.47 (1), p.143-152
• Main Authors: van der Heijden, M.H.T., Booms, G.H.R., Tanck, M.W.T., Rombout, J.H.W.M., Boon, J.H.
• Format: Article
• Language: English
• Subjects: ANGUILLA (GENERO); ANGUILLA (GENRE); ANGUILLA (GENUS); Anguilla anguilla; Animals; Anti-Infective Agents - pharmacology; Antimetabolites - pharmacology; Aquaculture; Aquaculture and Fisheries; Aquacultuur en Visserij; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Brackish; Bromodeoxyuridine - pharmacology; Concanavalin A - pharmacology; DNA - biosynthesis; DNA Replication - drug effects; DRUGS; Eels - immunology; Experimental Animal Morphology and Cell Biology; Experimentele diermorfologie en celbiologie; Female; Flow Cytometry - veterinary; Fluoroquinolones; Freshwater; IMMUNOGLOBULINE; IMMUNOGLOBULINS; Immunohistochemistry; INMUNOGLOBULINA; LECTINAS; LECTINE; LECTINS; Leerstoelgroep Aquacultuur en Visserij; LIPOPOLISACARIDOS; LIPOPOLYSACCHARIDE; LIPOPOLYSACCHARIDES; Lipopolysaccharides - pharmacology; Lymphocyte Activation - drug effects; Male; Marine; MEDICAMENT; MEDICAMENTOS; Quinolizines - pharmacology; Random Allocation; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; WIAS
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/0165-2427(94)05397-B

The influence of flumequine on mitogen induced lymphoid cell proliferation in European eels ( Anguilla anguilla L., 1758) was studied. For this purpose an in vivo test, using peroral drug administration followed by successive intraperitoneal injections with concanavalin A (ConA) or bacterial lipopolysaccharides (LPS) and 5-bromo-2′-deoxyuridine, was applied. Direct counting of proliferated cells in blood smears revealed that flumequine possesses mitogenic properties. A synergistic and an antagonistic effect of the drug was observed after LPS and ConA stimulation, respectively. Flow cytometric analysis of peripheral blood lymphoid cells showed a significant reduction of the mean proportion surface immunoglobulin positive cells in the flumequine-treated animals. It is concluded that flumequine enhances proliferation of lymphoid cells (probably surface immunoglobulin negative cells) in eel under the present experimental conditions.