Different capacities of carp leukocytes to encounter nitric oxide-mediated stress: a role for the intracellular reduced glutathione pool

Carp head kidney (HK) phagocytes can be stimulated by lipopolysaccharide (LPS) to produce nitric oxide (NO). High production of NO can suppress the carp immune system. Carp peripheral blood leukocytes (PBL) are highly susceptible but HK phagocytes are relatively resistant to the immunosuppressive ef...

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Bibliographic Details
Published in:Developmental and comparative immunology 2003-06, Vol.27 (6), p.555-568
Main Authors: Saeij, Jeroen P.J., van Muiswinkel, Willem B., van de Meent, Marian, Amaral, Catharina, Wiegertjes, Geert F.
Format: Article
Language:English
Subjects:
Animals
biosynthesis
Carp
Carps - physiology
Cell Division - physiology
cells
cellular glutathione
Cyprinidae
cyprinus-carpio
cytotoxicity
Fish
flow-cytometry
Freshwater
G6PD
gamma -glutamylcysteine synthetase
gene-expression
Glucosephosphate Dehydrogenase - genetics
Glucosephosphate Dehydrogenase - metabolism
Glutamate-Cysteine Ligase - genetics
Glutamate-Cysteine Ligase - metabolism
Glutathione
Glutathione - metabolism
head kidney
Leukocytes - physiology
manganese superoxide dismutase
MnSOD
Molecular Sequence Data
Nitric oxide
Nitric Oxide - metabolism
ornithine decarboxylase
Oxidative Stress - physiology
Phagocytes
RNA, Messenger
Superoxide Dismutase - genetics
Superoxide Dismutase - metabolism
Superoxides - metabolism
variability
γ-GCS
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Summary:Carp head kidney (HK) phagocytes can be stimulated by lipopolysaccharide (LPS) to produce nitric oxide (NO). High production of NO can suppress the carp immune system. Carp peripheral blood leukocytes (PBL) are highly susceptible but HK phagocytes are relatively resistant to the immunosuppressive effects of NO. This study demonstrates that the antioxidant glutathione plays an important role in the protection against nitrosative stress. Carp HK phagocytes, especially the neutrophilic granulocytes, contain higher levels of glutathione than PBL. Moreover, freshly isolated carp neutrophilic granulocytes have higher mRNA levels than PBL of glucose-6-phosphate dehydrogenase (G6PD), manganese superoxide dismutase (MnSOD) and γ-glutamylcysteine synthetase (γ-GCS). Since these molecules are part of the glutathione redox cycle, neutrophilic granulocytes have a higher capacity than PBL to maintain glutathione in a reduced state following nitrosative stress. When stimulated with LPS, neutrophilic granulocytes upregulate the expression of G6PD, MnSOD and γ-GCS.
ISSN:0145-305X
1879-0089
DOI:10.1016/S0145-305X(02)00158-1