Milking microalga Dunaliella salina for beta-carotene production in two-phase bioreactors

• Published in: Biotechnology and bioengineering 2004-03, Vol.85 (5), p.475-481
• Main Authors: Hejazi, M.A, Holwerda, E, Wijffels, R.H
• Format: Article
• Language: English
• Subjects: Algal culture (microalgae); beta Carotene - biosynthesis; beta Carotene - isolation & purification; beta-carotene; Biological and medical sciences; biological production; Bioreactors - microbiology; Biotechnology; Cell Culture Techniques - methods; Cell Division - physiology; Cell Survival; Chlorophyll - metabolism; Chlorophyta - cytology; Chlorophyta - metabolism; Chlorophyta - radiation effects; Dunaliella salina; extraction; Fundamental and applied biological sciences. Psychology; jet; Light; Methods. Procedures. Technologies; milking; two-phase bioreactors; β-carotene
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.10914

A new method was developed for production of beta-carotene from Dunaliella salina. Cells were grown in low light intensity and then transferred to a production bioreactor illuminated at a higher light intensity. It was a two-phase bioreactor consisting of an aqueous and a biocompatible organic phase. Mixing of the cells and extraction were performed by recirculation of the organic phase. Two experiments were performed. In the first experiment, bioreactors were operated at two different solvent recirculation rates of 150 and 200 mL min-1. The beta-carotene extraction rate increased significantly at the higher recirculation rate, without exerting any influence on cell number and viability. A second experiment was carried out at a recirculation rate (200 mL min-1) appropriate for the study of long-term production of beta-carotene. The results show that D. salina at high light intensity remained viable for a long period (>47 days) in the presence of a biocompatible organic phase; however, cell growth was very slow. beta-Carotene could be continuously extracted to the organic phase; the cells continued to produce beta-carotene and the extracted molecules were continuously reproduced. As a result, beta-carotene was continuously removed (milked) from the cells. beta-Carotene extraction efficiency in this system was >55%, and productivity was 2.45 mg m-2 day-1, much higher than that of commercial plants.