Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Comple...

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Bibliographic Details
Published in:Water science and technology 2003-01, Vol.48 (6), p.9-16
Main Authors: Cervantes, F J, Duong-Dac, T, Roest, K, Akkermans, A D L, Lettinga, G, Field, J A
Format: Article
Language:English
Subjects:
afvalwaterbehandeling
Anaerobic Digestion
anaerobic treatment
anaërobe behandeling
anthraquinone-2,6-disulfonate
Azo dyes
Bacteria
Bacteria, Anaerobic - physiology
Benzoquinones - metabolism
Bioreactors
Biotransformation
carbon-tetrachloride
chinonen
Consortia
dye reduction
electron-acceptors
Fatty acids
granules
humic substances
immobilisatie
Immobilization
Indicators and Reagents - metabolism
Inoculum
korrels
micro-organismen
microbial oxidation
Microorganisms
Models, Theoretical
Oxidation-Reduction
Population Dynamics
quinones
Reactors
recovery
redox mediators
reductie
reduction
respiration
Sewage - microbiology
slib
Sludge
sludges
Waste Disposal, Fluid - methods
waste water treatment
Xenobiotics
Xenobiotics - metabolism
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Summary:The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Complete reduction of AQDS was achieved by the granular sludge for a prolonged period in an anaerobic bioreactor provided with a mixture of volatile fatty acids as a substrate. Phylogenetic analysis revealed the enrichment and immobilization of AQDS-respiring bacteria appearing as dominant organisms in the microbial population of the AQDS-supplemented reactor, compared to a reactor control operated under methanogenic conditions. The consistent quinone-reducing capacity observed in the consortium indicates that it is feasible to apply quinone-reducing microorganisms in continuous bioreactors and this ability can potentially be important in wastewaters rich in humic substances. The quinone reducing activity could also be applied to accelerate the conversion of xenobiotics susceptible to reductive biotransformations such as azo dyes and polychlorinated compounds in continuous bioreactors.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2003.0345