Loading…
Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins
The application of the baculovirus-insect cell expression system for the production of integral membrane and secreted proteins is often more troublesome than for cytoplasmic proteins. One protein expressed at low levels in insect cells is the Theileria parva sporozoite surface protein p67. Theileria...
Saved in:
| Published in: | Journal of virological methods 2004-12, Vol.122 (1), p.113-118 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3 |
| container_end_page | 118 |
| container_issue | 1 |
| container_start_page | 113 |
| container_title | Journal of virological methods |
| container_volume | 122 |
| creator | Kaba, Stephen A. Salcedo, Adriana M. Wafula, Paul O. Vlak, Just M. van Oers, Monique M. |
| description | The application of the baculovirus-insect cell expression system for the production of integral membrane and secreted proteins is often more troublesome than for cytoplasmic proteins. One protein expressed at low levels in insect cells is the
Theileria parva sporozoite surface protein p67.
Theileria parva is a protozoan parasite, which causes the tick-transmitted disease East Coast fever in cattle. Baculovirus vectors were engineered to produce a secreted form of p67 by replacing the signal peptide of p67 with the honeybee mellitin signal sequence and deleting a putative membrane anchor from the C-terminus. Furthermore, the
chitinase and
v-cathepsin genes were deleted from the baculovirus expression vector in a bacmid setup, allowing broad scale application of this novel vector. Deletion of the
chitinase and
v-cathepsin gene had a positive effect on the integrity of both the intracellular and secreted recombinant protein. |
| doi_str_mv | 10.1016/j.jviromet.2004.07.006 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_wagen</sourceid><recordid>TN_cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_333713</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0166093404002149</els_id><sourcerecordid>17769576</sourcerecordid><originalsourceid>FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhSMEokPhL1TZwC7Bj7EdswIVaJEqsYG15cfN1KPECbaTqv--jmaAZTe-lvWd42OfqrrCqMUI84_H9rj6OI2QW4LQvkWiRYi_qHa4E7JBstu_rHYF5GVP9xfVm5SOCCEmKH1dXWC27zpOul0Vv8IKwzSPEHI99bWu7b3PPugEtQ6uXhur8z3MyYc6wEFnv0JttB29q_sp1n6c47SCq33IcIg-P24uCWyEXE4j2Gk0xa64FzCDD-lt9arXQ4J353lZ_f7-7df1bXP38-bH9Ze7xjIic-MMthy5zhAtCaccMdJzI6VklBNrkaHISUpQz3UvO-MwN6J3QmjDMGHa0cvq08n3QR8g-FAWFXS0PqlJezV4E3V8VA9LVGHYxryYpCilAtMi_nASl9R_FkhZjT5ZGAYdYFqS4lwK3kn8LIiF4JIJXkB-Am2cUorQqzn6cUuAkdo6VUf1t1O1daqQUKXTIrw637CYEdx_2bnEArw_AzpZPfRRh-2R_zhOCGNCFu7ziYPy66uHqJL1ECw4X3rKyk3-uSxPvT3HOg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17769576</pqid></control><display><type>article</type><title>Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins</title><source>Elsevier</source><creator>Kaba, Stephen A. ; Salcedo, Adriana M. ; Wafula, Paul O. ; Vlak, Just M. ; van Oers, Monique M.</creator><creatorcontrib>Kaba, Stephen A. ; Salcedo, Adriana M. ; Wafula, Paul O. ; Vlak, Just M. ; van Oers, Monique M.</creatorcontrib><description>The application of the baculovirus-insect cell expression system for the production of integral membrane and secreted proteins is often more troublesome than for cytoplasmic proteins. One protein expressed at low levels in insect cells is the
Theileria parva sporozoite surface protein p67.
Theileria parva is a protozoan parasite, which causes the tick-transmitted disease East Coast fever in cattle. Baculovirus vectors were engineered to produce a secreted form of p67 by replacing the signal peptide of p67 with the honeybee mellitin signal sequence and deleting a putative membrane anchor from the C-terminus. Furthermore, the
chitinase and
v-cathepsin genes were deleted from the baculovirus expression vector in a bacmid setup, allowing broad scale application of this novel vector. Deletion of the
chitinase and
v-cathepsin gene had a positive effect on the integrity of both the intracellular and secreted recombinant protein.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2004.07.006</identifier><identifier>PMID: 15488628</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Baculoviridae - enzymology ; Baculoviridae - genetics ; Baculovirus ; Baculovirus expression system ; baculovirus system ; Biological and medical sciences ; Cathepsins - genetics ; cattle ; Chitinase ; Chitinases - genetics ; East Coast fever ; escherichia-coli ; expression ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Viral ; Genetic Vectors ; Honeybee mellitin signal ; immunity ; immunogenicity ; insect cells ; Melitten - genetics ; Melitten - physiology ; Microbiology ; p67 ; Protein Sorting Signals ; Protozoan Proteins - genetics ; Protozoan Proteins - metabolism ; Recombinant Proteins - metabolism ; Techniques used in virology ; Theileria parva ; v-cathepsin ; vaccine antigen ; Virology ; virus</subject><ispartof>Journal of virological methods, 2004-12, Vol.122 (1), p.113-118</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3</citedby><cites>FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16225579$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15488628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaba, Stephen A.</creatorcontrib><creatorcontrib>Salcedo, Adriana M.</creatorcontrib><creatorcontrib>Wafula, Paul O.</creatorcontrib><creatorcontrib>Vlak, Just M.</creatorcontrib><creatorcontrib>van Oers, Monique M.</creatorcontrib><title>Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>The application of the baculovirus-insect cell expression system for the production of integral membrane and secreted proteins is often more troublesome than for cytoplasmic proteins. One protein expressed at low levels in insect cells is the
Theileria parva sporozoite surface protein p67.
Theileria parva is a protozoan parasite, which causes the tick-transmitted disease East Coast fever in cattle. Baculovirus vectors were engineered to produce a secreted form of p67 by replacing the signal peptide of p67 with the honeybee mellitin signal sequence and deleting a putative membrane anchor from the C-terminus. Furthermore, the
chitinase and
v-cathepsin genes were deleted from the baculovirus expression vector in a bacmid setup, allowing broad scale application of this novel vector. Deletion of the
chitinase and
v-cathepsin gene had a positive effect on the integrity of both the intracellular and secreted recombinant protein.</description><subject>Baculoviridae - enzymology</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Baculovirus expression system</subject><subject>baculovirus system</subject><subject>Biological and medical sciences</subject><subject>Cathepsins - genetics</subject><subject>cattle</subject><subject>Chitinase</subject><subject>Chitinases - genetics</subject><subject>East Coast fever</subject><subject>escherichia-coli</subject><subject>expression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genetic Vectors</subject><subject>Honeybee mellitin signal</subject><subject>immunity</subject><subject>immunogenicity</subject><subject>insect cells</subject><subject>Melitten - genetics</subject><subject>Melitten - physiology</subject><subject>Microbiology</subject><subject>p67</subject><subject>Protein Sorting Signals</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - metabolism</subject><subject>Recombinant Proteins - metabolism</subject><subject>Techniques used in virology</subject><subject>Theileria parva</subject><subject>v-cathepsin</subject><subject>vaccine antigen</subject><subject>Virology</subject><subject>virus</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhSMEokPhL1TZwC7Bj7EdswIVaJEqsYG15cfN1KPECbaTqv--jmaAZTe-lvWd42OfqrrCqMUI84_H9rj6OI2QW4LQvkWiRYi_qHa4E7JBstu_rHYF5GVP9xfVm5SOCCEmKH1dXWC27zpOul0Vv8IKwzSPEHI99bWu7b3PPugEtQ6uXhur8z3MyYc6wEFnv0JttB29q_sp1n6c47SCq33IcIg-P24uCWyEXE4j2Gk0xa64FzCDD-lt9arXQ4J353lZ_f7-7df1bXP38-bH9Ze7xjIic-MMthy5zhAtCaccMdJzI6VklBNrkaHISUpQz3UvO-MwN6J3QmjDMGHa0cvq08n3QR8g-FAWFXS0PqlJezV4E3V8VA9LVGHYxryYpCilAtMi_nASl9R_FkhZjT5ZGAYdYFqS4lwK3kn8LIiF4JIJXkB-Am2cUorQqzn6cUuAkdo6VUf1t1O1daqQUKXTIrw637CYEdx_2bnEArw_AzpZPfRRh-2R_zhOCGNCFu7ziYPy66uHqJL1ECw4X3rKyk3-uSxPvT3HOg</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Kaba, Stephen A.</creator><creator>Salcedo, Adriana M.</creator><creator>Wafula, Paul O.</creator><creator>Vlak, Just M.</creator><creator>van Oers, Monique M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>QVL</scope></search><sort><creationdate>20041201</creationdate><title>Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins</title><author>Kaba, Stephen A. ; Salcedo, Adriana M. ; Wafula, Paul O. ; Vlak, Just M. ; van Oers, Monique M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Baculoviridae - enzymology</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Baculovirus expression system</topic><topic>baculovirus system</topic><topic>Biological and medical sciences</topic><topic>Cathepsins - genetics</topic><topic>cattle</topic><topic>Chitinase</topic><topic>Chitinases - genetics</topic><topic>East Coast fever</topic><topic>escherichia-coli</topic><topic>expression</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Viral</topic><topic>Genetic Vectors</topic><topic>Honeybee mellitin signal</topic><topic>immunity</topic><topic>immunogenicity</topic><topic>insect cells</topic><topic>Melitten - genetics</topic><topic>Melitten - physiology</topic><topic>Microbiology</topic><topic>p67</topic><topic>Protein Sorting Signals</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - metabolism</topic><topic>Recombinant Proteins - metabolism</topic><topic>Techniques used in virology</topic><topic>Theileria parva</topic><topic>v-cathepsin</topic><topic>vaccine antigen</topic><topic>Virology</topic><topic>virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaba, Stephen A.</creatorcontrib><creatorcontrib>Salcedo, Adriana M.</creatorcontrib><creatorcontrib>Wafula, Paul O.</creatorcontrib><creatorcontrib>Vlak, Just M.</creatorcontrib><creatorcontrib>van Oers, Monique M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>NARCIS:Publications</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaba, Stephen A.</au><au>Salcedo, Adriana M.</au><au>Wafula, Paul O.</au><au>Vlak, Just M.</au><au>van Oers, Monique M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>122</volume><issue>1</issue><spage>113</spage><epage>118</epage><pages>113-118</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>The application of the baculovirus-insect cell expression system for the production of integral membrane and secreted proteins is often more troublesome than for cytoplasmic proteins. One protein expressed at low levels in insect cells is the
Theileria parva sporozoite surface protein p67.
Theileria parva is a protozoan parasite, which causes the tick-transmitted disease East Coast fever in cattle. Baculovirus vectors were engineered to produce a secreted form of p67 by replacing the signal peptide of p67 with the honeybee mellitin signal sequence and deleting a putative membrane anchor from the C-terminus. Furthermore, the
chitinase and
v-cathepsin genes were deleted from the baculovirus expression vector in a bacmid setup, allowing broad scale application of this novel vector. Deletion of the
chitinase and
v-cathepsin gene had a positive effect on the integrity of both the intracellular and secreted recombinant protein.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15488628</pmid><doi>10.1016/j.jviromet.2004.07.006</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 2004-12, Vol.122 (1), p.113-118 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_333713 |
| source | Elsevier |
| subjects | Baculoviridae - enzymology Baculoviridae - genetics Baculovirus Baculovirus expression system baculovirus system Biological and medical sciences Cathepsins - genetics cattle Chitinase Chitinases - genetics East Coast fever escherichia-coli expression Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Genetic Vectors Honeybee mellitin signal immunity immunogenicity insect cells Melitten - genetics Melitten - physiology Microbiology p67 Protein Sorting Signals Protozoan Proteins - genetics Protozoan Proteins - metabolism Recombinant Proteins - metabolism Techniques used in virology Theileria parva v-cathepsin vaccine antigen Virology virus |
| title | Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T15%3A04%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wagen&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20chitinase%20and%20v-cathepsin%20negative%20bacmid%20for%20improved%20integrity%20of%20secreted%20recombinant%20proteins&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Kaba,%20Stephen%20A.&rft.date=2004-12-01&rft.volume=122&rft.issue=1&rft.spage=113&rft.epage=118&rft.pages=113-118&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/j.jviromet.2004.07.006&rft_dat=%3Cproquest_wagen%3E17769576%3C/proquest_wagen%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c529t-db1c60d8b2a92636052f6b9995362cc0b30d9320f6af98bd16b7fd77ab5125ad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17769576&rft_id=info:pmid/15488628&rfr_iscdi=true |