Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr)

• Published in: Plant cell reports 2005-11, Vol.24 (9), p.507-512
• Main Authors: Shan, Z, Raemaker, K, Tzitzikas, E.N, Ma, Z, Visser, R.G.F
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; cotyledonary-node; cotyledons; Culture Media; Eukaryotic cell cultures; field crops; Fundamental and applied biological sciences. Psychology; Genotypes; Glycine max; Glycine max - embryology; growth; Growth regulators; intact seedlings; Methods. Procedures. Technologies; micropropagation; Miscellaneous; organogenesis; pea pisum-sativum; Plant cells and fungal cells; plant regeneration; seed germination; seedling growth; Seeds; Seeds - growth & development; shoot formation; somatic embryogenesis; somatic embryos; Soybeans; thiazoles; thidiazuron; tissue
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-005-0971-7

A highly efficient, repetitive system of organogenesis was developed in soybean. Seeds of soybean cv. 'White hilum' pretreated with TDZ formed multiple bud tissue(s) (MBT) at the cotyledonary nodes. MBT initiation occurred only if the axillary buds were not removed from the cotyledonary node. The best MBT formation was achieved by pretreating the seeds for 1 week on medium supplemented with 0.1 mg/l TDZ, followed by culture of the cotyledonary node on medium supplemented with 0.5 mg/l BA for 4 weeks. Culture of the MBT on medium supplemented with 0.1 mg/l TDZ resulted in the proliferation of MBT. MBT was maintained in this way for 12 months. Three hundred thirty six shoots were obtained when 1 g of MBT was subcultured on medium supplemented with 0.5 mg/l BA. Plants were rooted on medium without growth regulators. The regenerated plants grew normally in the greenhouse. Unfortunately, they did not set seeds because of the long-day conditions during growth. This system was successfully applied in three other genotypes.