Simultaneous quantification of differently glycosylated, acetylated, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection

A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains,...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-04, Vol.1072 (2), p.185-193
Main Authors: Decroos, K, Vincken, J.P, Heng, L, Bakker, R, Gruppen, H, Verstraete, W
Format: Article
Language:English
Subjects:
Acetylation
Agronomy. Soil science and plant productions
Biological and medical sciences
Chemical constitution
Chromatography, High Pressure Liquid - methods
Economic plant physiology
evaporative light scattering detection
food
Fundamental and applied biological sciences. Psychology
ginseng
glycine-max
Glycosylation
group-a saponin
in-vitro
Light
light scattering
Mass Spectrometry
Reproducibility of Results
reversed-phase high performance liquid chromatography
sapogenol
saponins
Saponins - analysis
Scattering, Radiation
soy
soyasapogenol-a
soyasaponins
soybean seed
soybeans
varieties
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Summary:A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains, occurring in soy. Group A soyasaponins in different degrees of acetylation, as well as group B soyasaponins in both their 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated and non-conjugated forms could be separated and quantified using authentic soyasaponin standards, in one single run. The method was tested by the determination of the soyasaponin content and composition of eight soygerm samples of different origin. Differences in the composition and the degree of acetylation of the group A soyasaponins were observed among these samples. The group B soyasaponins showed much less variability and they were mainly present in their DDMP-conjugated form.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.03.021