A monoclonal antibody-based ELISA allows discrimination between responses induced by bovine herpesvirus subtypes 1 (BoHV-1.1) and 2 (BoHV-1.2)

• Published in: Journal of virological methods 2005-11, Vol.129 (2), p.191-193
• Main Authors: Spilki, Fernando Rosado, Esteves, Paulo Augusto, Silva, Alessandra D’Ávila da, Franco, Ana Cláudia, Rijsewijk, Frans A.M., Roehe, Paulo Michel
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Antibodies, Viral; Antibody Specificity; bhv-1; Biological and medical sciences; Cattle; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Herpes Simplex - immunology; Herpesviridae Infections - immunology; Herpesvirus; Herpesvirus 1, Bovine - immunology; Herpesvirus 2, Bovine - immunology; Immunization; infections; Infectious Bovine Rhinotracheitis - immunology; Microbiology; restriction-endonuclease; Sensitivity and Specificity; Techniques used in virology; type-1; Viral Proteins - immunology; Virology
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2005.06.008

Bovine herpesvirus type 1 (BoHV-1) has distinct subtypes according to genomic characterization. Immune responses induced by BoHV-1 subtype 1 (BoHV-1.1) are not distinguishable from those induced by BoHV-1 subtype 2 (BoHV-1.2) through conventional serological methods. In the present report, an enzyme linked immunosorbent assay is described that allows discrimination between immune responses in cattle immunized with either subtype, based on a monoclonal antibody that recognizes specifically the amino-terminal region of glycoprotein C (gC) on BoHV-1.1 strains, thus not reacting with BoHV-1.2a. The test displayed a sensitivity of 92%, specificity of 90% and a good correlation with serum neutralization tests on samples from BoHV-1.1-immunized calves ( κ = 0.799). The test may be useful to provide new insights into the roles played by each of these two subtypes in the epidemiology of BoHV-1 infections.