Progenies of allotriploids of Oriental x Asiatic lilies (Lilium) examined by GISH analysis

With the aim of utilizing allotriploid (2n = 3x = 36) lily hybrids (Lilium) in introgression breeding, different types of crosses were made. First, using diploid Asiatic lilies (2n = 2x = 24), reciprocal crosses (3x - 2x and 2x - 3x) were made with allotriploid hybrids (AOA) obtained by backcrosses...

Full description

Saved in:
Bibliographic Details
Published in:Euphytica 2006-09, Vol.151 (2), p.243-250
Main Authors: Barba-Gonzalez, Rodrigo, Van Silfhout, Alex A, Visser, Richard GF, Ramanna, Munikote S, Van Tuyl, Jaap M
Format: Article
Language:English
Subjects:
angiosperms
crosses
culture
liliaceae
Lilium
lily hybrids
narcissus
nuclear-dna amounts
recombination
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:With the aim of utilizing allotriploid (2n = 3x = 36) lily hybrids (Lilium) in introgression breeding, different types of crosses were made. First, using diploid Asiatic lilies (2n = 2x = 24), reciprocal crosses (3x - 2x and 2x - 3x) were made with allotriploid hybrids (AOA) obtained by backcrosses of F sub(1) Oriental x Asiatic hybrids (OA) to Asiatic cultivars (A). Secondly, the AOA allotriploids were crossed with allotetraploid (OAOA, 2n = 4x = 48), in 3x - 4x combination. Finally, the AOA allotriploids where crossed to 2n gamete producer F sub(1) OA hybrids (3x - 2x (2n)). Two types of triploids were used as parents in the different types of crosses, derived from: (a) mitotic polyploidization and (b) sexual polyploidization. Ploidy level of the progeny was determined by estimating the DNA values through flowcytometry as well as chromosome counting. The aneuploid progeny plants from 3x - 2x and reciprocal crosses had approximate diploid levels and in 3x - 4x crosses and 3x - 2x (2n) the progeny had approximate tetraploid levels. Balanced euploid gametes (x, 2x and 3x) were formed in the AOA genotypes. Recombinant chromosomes were found in the progenies of all crosses, except in the case of 2x - 3x crosses through genomic in situ hybridization (GISH) analyses. Recombinant chromosomes occurred in the F sub(1) OA hybrid when the triploid AOA hybrid was derived through sexual polyploidization, but not through mitotic polyploidization with two exceptions. Those recombinant chromosomes were transmitted to the progenies in variable frequencies.
ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-006-9148-x