Targeted transcript mapping for agronomic traits in potato

A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness of tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments with a polymorphic segregation pat...

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Bibliographic Details
Published in:Journal of experimental botany 2007-09, Vol.58 (11), p.2761-2774
Main Authors: Fernández-del-Carmen, Asun, Celis-Gamboa, Carolina, Visser, Richard G. F., Bachem, Christian W. B.
Format: Article
Language:English
Subjects:
20-oxidase gene
Agronomy. Soil science and plant productions
Amplified Fragment Length Polymorphism Analysis
Biological and medical sciences
Bulked segregant analysis
cDNA-AFLP
Chromosomes
Chromosomes, Artificial, Bacterial
Chromosomes, Plant
Contig Mapping
differential gene-expression
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Genes
Genes, Plant
Genetic mapping
Genetic Markers
Genetics and breeding of economic plants
Genotypes
gibberellin
in-vitro
kaurenoic acid oxidase
late blight
linkage maps
Pest resistance
Phenotype
phytochrome-b
Plant pathogens
Plant Tubers - genetics
Plants
Polymorphism, Genetic
potato tuberization
QTL
Quantitative Trait Loci
Research Papers
RNA, Messenger - metabolism
Segregation
Sequence Analysis, DNA
Solanum tuberosum
Solanum tuberosum - genetics
Solanum tuberosum - growth & development
transcript mapping
tuber development
Tubers
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness of tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments with a polymorphic segregation pattern between early and late tuberizing bulks. Most of the identified transcripts mapped to chromosomes 5 (19 markers) and 12 (eight markers) of the paternal map. Quantitative trait locus (QTL) mapping of tuberization time also identified earliness QTLs on these two chromosomes. A potato bacterial artificial chromosome (BAC) library was screened with four of the markers linked to the main QTL. BAC contigs containing the markers showing the highest association with the trait have been identified. One of these contigs has been anchored to chromosome 5 on an ultradense genetic map of potato, which could be used as a starting point for map-based cloning of genes associated with earliness.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erm140