Preparative Isolation and Separation Procedure for Ginkgolides A, B, C, and J and Bilobalide

A simple preparative method for the isolation and purification of ginkgolides A, B, C, and J and bilobalide (ginkgo terpene trilactones) was developed. As starting material, a commercially available leaf extract from Ginkgo biloba containing ≥ 6% ginkgo terpene trilactones was used. After a partitio...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 1997-07, Vol.60 (7), p.735-738
Main Authors: van Beek, Teris A, Lelyveld, Gerrit P
Format: Article
Language:English
Subjects:
Biological and medical sciences
CHEMICAL COMPOSITION
CHEMICAL STRUCTURE
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
EPS
ESPECTROMETRIA
ESTRUCTURA QUIMICA
FEUILLE
General pharmacology
GINKGO BILOBA
HOJAS
ISOLATION TECHNIQUES
Laboratorium voor Organische chemie
Laboratory for Organic Chemistry
LACTONAS
LACTONE
LACTONES
LEAVES
Medical sciences
Organic Chemistry
Organische Chemie
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
PURIFICACION
PURIFICATION
SPECTRAL ANALYSIS
SPECTROMETRIE
SPECTROMETRY
STRUCTURE CHIMIQUE
TECHNIQUE DE L'ISOLEMENT
TECNICAS DE AISLAMIENTO
TRITERPENOIDE
TRITERPENOIDOS
TRITERPENOIDS
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Summary:A simple preparative method for the isolation and purification of ginkgolides A, B, C, and J and bilobalide (ginkgo terpene trilactones) was developed. As starting material, a commercially available leaf extract from Ginkgo biloba containing ≥ 6% ginkgo terpene trilactones was used. After a partition step with EtOAc, the enriched intermediate extract was separated into the individual terpenes by medium-pressure liquid chromatography on silica impregnated with 6.5% NaOAc with a gradient from petroleum ether−EtOAc to EtOAc−MeOH. After recrystallization from H2O−MeOH, all five terpenes could be isolated in high purity. After a selective extraction with H2O, leaves could also be used as a starting material.
ISSN:0163-3864
1520-6025
DOI:10.1021/np970060r