A simplified method for the determination of taxanes in yew needles by reversed-phase (C18) high pressure liquid chromatography

An analytical procedure for the large‐scale qualitative and quantitative screening of paclitaxel (Taxol®) and related neural taxanes in yew needles has been developed. The needles were selectively extracted with ethanol:water:acetic acid (80:19:1) and the resulting extract was purified from chloroph...

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Bibliographic Details
Published in:Phytochemical analysis 1997-11, Vol.8 (6), p.286-293
Main Authors: van Rozendaal, Erik L. M., Lelyveld, Gerrit P., van Beek, Teris A.
Format: Article
Language:English
Subjects:
EPS
Extrulet
high pressure liquid chromatography
Laboratorium voor Organische chemie
Laboratory for Organic Chemistry
Organic Chemistry
Organische Chemie
paclitaxel
quantitative determination
Taxaceae
taxanes
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Summary:An analytical procedure for the large‐scale qualitative and quantitative screening of paclitaxel (Taxol®) and related neural taxanes in yew needles has been developed. The needles were selectively extracted with ethanol:water:acetic acid (80:19:1) and the resulting extract was purified from chlorophyll, 3,5‐dimethyoxy phenol, taxine alkaloids and cinnamoyl taxanes, by means of small‐scale partition chromatography on Extrulet®. After concentration, the taxanes were determined by high pressure chromatography on a C18 column with acetonitrile;water as eluent and ultra violet detection at 227 and 280 nm. Cinnamic acid was used as an internal standard. Various aspects such as extraction efficiency, recovery, peak purity, detection limits, robustness and reproducibility of the method have been evaluated. The method is very labour efficient with only 20 min of manpower input per sample. © 1997 John Wiley & Sons, Ltd.
ISSN:0958-0344
1099-1565
DOI:10.1002/(SICI)1099-1565(199711/12)8:6<286::AID-PCA374>3.0.CO;2-G