Transposition of a fungal mite through the action of a Tc1-like transposase

The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since mimp1 has no coding capacity, it...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 2007, Vol.175
Main Authors: Dufresne, M, Hua-Van, A, Abd el Wahab, H, M'Barek, S., Ben, Kema, G.H.J, Daboussi, M.J
Format: Article
Language:
Subjects:
arabidopsis-thaliana
aspergillus-nidulans
caenorhabditis-elegans
dna transposon
emigrant family
fusarium-oxysporum
genes
genome sequence
neurospora-crassa
rice
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mimp1 element previously identified in the ascomycete fungus Fusarium oxysporum has hallmarks of miniature inverted-repeat transposable elements (MITEs): short size, terminal inverted repeats (TIRs), structural homogeneity, and a stable secondary structure. Since mimp1 has no coding capacity, its mobilization requires a transposase-encoding element. On the basis of the similarity of TIRs and target-site preference with the autonomous Tcl-like element impala, together with a correlated distribution of both elements among the Fusarium genus, we investigated the ability of mimp1 to jump upon expression of the impala transposase provided in trans. Under these conditions, we present evidence that mimp1 transposes by a cut-and-paste mechanism into TA dinucleoticles, which are duplicated upon insertion. Our results also show that mimp1 reinserts very frequently in genic regions for at least one-third of the cases. We also show that the mimp1/impala, double-component system is fully functional in the heterologous species F. graminearum, allowing the development of a highly efficient tool for gene tagging in filamentous fungi.
ISSN:0016-6731
1943-2631