Rhizoplane colonisation of peas by Rhizobium leguminosarum bv. viceae and a deleterious Pseudomonas putida

Pseudomonas putida strain Å313, a deleterious rhizosphere bacterium, reduced pea nitrogen content when inoculated alone or in combination with Rhizobium leguminosarum bv. viceae on plants in the presence of soil under greenhouse conditions. When plants were grown gnotobiotically in liquid media, mix...

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Bibliographic Details
Published in:FEMS microbiology ecology 2005-03, Vol.52 (1), p.71-78
Main Authors: Berggren, Ingela, Alström, Sadhna, van Vuurde, J.W.L., Mårtensson, Anna M.
Format: Article
Language:English
Subjects:
Analysis of Variance
azospirillum-brasilense
Bacteriology
Biological and medical sciences
Biological nitrogen fixation
Colonization
Deleterious rhizosphere bacteria
Ecology
fluorescent pseudomonas
Fundamental and applied biological sciences. Psychology
Gnotobiotic
growth promoting rhizobacteria
Immunofluorescence
Inoculation
Inoculum
Microbiology
Microscopy, Fluorescence
Miscellaneous
Nitrogen - metabolism
nitrogen-fixation
Nodulation
Nodules
pathogenic fungi
Peas
phaseolus-vulgaris
Pisum sativum
Pisum sativum - metabolism
Pisum sativum - microbiology
pisum-sativum l
Plant Roots - microbiology
plant-growth
Pseudomonads
Pseudomonas putida
Pseudomonas putida - pathogenicity
Pseudomonas putida - physiology
Rhizobium leguminosarum
Rhizobium leguminosarum - physiology
Rhizoplane
Rhizosphere
Root colonisation
root colonization
Root hairs
Seedlings
Soil conditions
Symbiosis
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Summary:Pseudomonas putida strain Å313, a deleterious rhizosphere bacterium, reduced pea nitrogen content when inoculated alone or in combination with Rhizobium leguminosarum bv. viceae on plants in the presence of soil under greenhouse conditions. When plants were grown gnotobiotically in liquid media, mixed inocula of Å313 and rhizobia gave a higher proportion of small evenly distributed nodules when compared with a single rhizobial inoculation. In addition, the rhizobial root establishment was reduced by Å313 irrespective of inoculum density, indicating that Å313 has the capacity to interact with the early rhizobial infection process. When pea seedlings were simultaneously inoculated with Å313 and rhizobia, Å313 colonised the root hairs to the same extent as the rhizobia, according to analysis by immunofluorescence microscopy. This suggests that the root hair colonisation trait of P. putida interferes with the onset of the symbiotic process.
ISSN:0168-6496
1574-6941
DOI:10.1016/j.femsec.2004.10.013