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An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging
We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes...
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| Published in: | Plant physiology (Bethesda) 1997-05, Vol.114 (1), p.383-389 |
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| Main Authors: | , , |
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| Language: | English |
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| container_end_page | 389 |
| container_issue | 1 |
| container_start_page | 383 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 114 |
| creator | Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.) Tikhonov, A.N Hoekstra, F.A |
| description | We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy and the broadening agent ferricyanide. The line-height ratio between the water and lipid components in the electron paramagnetic resonance spectra of 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (R value) allowed for the quantitative assessment of the plasma membrane permeability in small samples, enabling separate studies of the axis, scutellum, aleurone layer, and starchy endosperm tissue. High R values corresponded to low permeability and vice versa. Starchy endosperm cells had completely permeable plasma membranes even in mature, viable seeds. The loss of germinability with aging coincided with a considerably increased plasma membrane permeability of the embryo axis cells, but not of the scutellum and aleurone layer cells. The threshold R value for the individual axes associated with viability loss was established at 5 to 6, with the total ranging from 0 to more than 12. We suggest that the R value of an individual axis is the result of contributions from all individual cells, each of them characterized by a different permeability. The loss of viability, therefore, corresponds to the accumulation of cells having permeability above a critical level |
| doi_str_mv | 10.1104/pp.114.1.383 |
| format | article |
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(Russian Academy of Sciences, Moscow, Russia.) ; Tikhonov, A.N ; Hoekstra, F.A</creator><creatorcontrib>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.) ; Tikhonov, A.N ; Hoekstra, F.A</creatorcontrib><description>We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy and the broadening agent ferricyanide. The line-height ratio between the water and lipid components in the electron paramagnetic resonance spectra of 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (R value) allowed for the quantitative assessment of the plasma membrane permeability in small samples, enabling separate studies of the axis, scutellum, aleurone layer, and starchy endosperm tissue. High R values corresponded to low permeability and vice versa. Starchy endosperm cells had completely permeable plasma membranes even in mature, viable seeds. The loss of germinability with aging coincided with a considerably increased plasma membrane permeability of the embryo axis cells, but not of the scutellum and aleurone layer cells. The threshold R value for the individual axes associated with viability loss was established at 5 to 6, with the total ranging from 0 to more than 12. We suggest that the R value of an individual axis is the result of contributions from all individual cells, each of them characterized by a different permeability. The loss of viability, therefore, corresponds to the accumulation of cells having permeability above a critical level</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.114.1.383</identifier><identifier>PMID: 12223711</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>AGING ; Aleurone layer ; APTITUD PARA LA CONSERVACION ; APTITUDE A LA CONSERVATION ; Cell membranes ; CELL STRUCTURE ; ELECTRON PARAMAGNETIC RESONANCE SPECTROS ; Embryos ; Endosperm ; ENVEJECIMIENTO ; EPS ; ESPECTROSCOPIA ESR ; ESR SPECTROSCOPY ; ESTRUCTURA CELULAR ; FISIOLOGIA POSTCOSECHA ; GERMINACION ; GERMINATION ; GERMOPLASMA ; GERMPLASM ; KEEPING QUALITY ; Laboratorium voor Plantenfysiologie ; Laboratory of Plant Physiology ; Lipids ; LONGEVITE DES SEMENCES ; MEASUREMENT ; MEDICION ; MESURE ; PERMEABILIDAD ; PERMEABILITE ; PERMEABILITY ; PHYSIOLOGIE APRES RECOLTE ; Plants ; PLASMA MEMBRANES ; POSTHARVEST PHYSIOLOGY ; SEED ; SEED LONGEVITY ; Seeds ; SEMENCE ; SEMILLAS ; SPECTROSCOPIE RSE ; STORAGE LIFE ; STRUCTURE CELLULAIRE ; TRITICUM ; TRITICUM AESTIVUM ; VIABILIDAD ; VIABILIDAD DE LA SEMILLA ; VIABILITE ; VIABILITY ; VIEILLISSEMENT ; Wheat ; Whole Plant, Environmental, and Stress Physiology</subject><ispartof>Plant physiology (Bethesda), 1997-05, Vol.114 (1), p.383-389</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-5510daafc1b5ceb3dc6195e3154d93bf46e7828572436f888097a6e0e4776d683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277715$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277715$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12223711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.)</creatorcontrib><creatorcontrib>Tikhonov, A.N</creatorcontrib><creatorcontrib>Hoekstra, F.A</creatorcontrib><title>An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy and the broadening agent ferricyanide. The line-height ratio between the water and lipid components in the electron paramagnetic resonance spectra of 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (R value) allowed for the quantitative assessment of the plasma membrane permeability in small samples, enabling separate studies of the axis, scutellum, aleurone layer, and starchy endosperm tissue. High R values corresponded to low permeability and vice versa. Starchy endosperm cells had completely permeable plasma membranes even in mature, viable seeds. The loss of germinability with aging coincided with a considerably increased plasma membrane permeability of the embryo axis cells, but not of the scutellum and aleurone layer cells. The threshold R value for the individual axes associated with viability loss was established at 5 to 6, with the total ranging from 0 to more than 12. We suggest that the R value of an individual axis is the result of contributions from all individual cells, each of them characterized by a different permeability. The loss of viability, therefore, corresponds to the accumulation of cells having permeability above a critical level</description><subject>AGING</subject><subject>Aleurone layer</subject><subject>APTITUD PARA LA CONSERVACION</subject><subject>APTITUDE A LA CONSERVATION</subject><subject>Cell membranes</subject><subject>CELL STRUCTURE</subject><subject>ELECTRON PARAMAGNETIC RESONANCE SPECTROS</subject><subject>Embryos</subject><subject>Endosperm</subject><subject>ENVEJECIMIENTO</subject><subject>EPS</subject><subject>ESPECTROSCOPIA ESR</subject><subject>ESR SPECTROSCOPY</subject><subject>ESTRUCTURA CELULAR</subject><subject>FISIOLOGIA POSTCOSECHA</subject><subject>GERMINACION</subject><subject>GERMINATION</subject><subject>GERMOPLASMA</subject><subject>GERMPLASM</subject><subject>KEEPING QUALITY</subject><subject>Laboratorium voor Plantenfysiologie</subject><subject>Laboratory of Plant Physiology</subject><subject>Lipids</subject><subject>LONGEVITE DES SEMENCES</subject><subject>MEASUREMENT</subject><subject>MEDICION</subject><subject>MESURE</subject><subject>PERMEABILIDAD</subject><subject>PERMEABILITE</subject><subject>PERMEABILITY</subject><subject>PHYSIOLOGIE APRES RECOLTE</subject><subject>Plants</subject><subject>PLASMA MEMBRANES</subject><subject>POSTHARVEST PHYSIOLOGY</subject><subject>SEED</subject><subject>SEED LONGEVITY</subject><subject>Seeds</subject><subject>SEMENCE</subject><subject>SEMILLAS</subject><subject>SPECTROSCOPIE RSE</subject><subject>STORAGE LIFE</subject><subject>STRUCTURE CELLULAIRE</subject><subject>TRITICUM</subject><subject>TRITICUM AESTIVUM</subject><subject>VIABILIDAD</subject><subject>VIABILIDAD DE LA SEMILLA</subject><subject>VIABILITE</subject><subject>VIABILITY</subject><subject>VIEILLISSEMENT</subject><subject>Wheat</subject><subject>Whole Plant, Environmental, and Stress Physiology</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpVUk2P1SAUJUbjPJ_uXKph6cI-odACi1lMJn4lk7jQWRNKb_uYtFCh9eX9e2n6MuqGc8M9h3tyLgi9puRAKeEfpykjP9ADk-wJ2tGKlUVZcfkU7QjJNZFSXaEXKT0QQiij_Dm6omVZMkHpDrU3HsMAdo7B48lEM5rew-wsjpCCN94CTpPzxRRDk8t5ac84dHiEsYnGQzFBHME0bnDzGduj8T0kfHLzESeAFpve-f4letaZIcGrC-7R_edPP2-_Fnffv3y7vbkrbEXJXFT5bI3pLG0qCw1rbU1VBYxWvFWs6XgNQpayEiVndSelJEqYGghwIeq2lmyP1PbuyfTg82Dw2ptoXdLBOD24bDme9WmJ2g8rTEuTNBOiVFl7vWnz3QitBT9HM-gpunHVrPr_O94ddR9-a1rJHGrWv7_oY_i1QJr16JKFYcghhSVpKivFhGJ8tflho9oYUorQPU6hRK871dOUkWuq804z_d2_zv6SL0vMhDcb4SHNIT72eSmEyN9hj95u7c4EbfqY07j_QZUShKvshv0BjC-y6g</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.)</creator><creator>Tikhonov, A.N</creator><creator>Hoekstra, F.A</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>QVL</scope></search><sort><creationdate>19970501</creationdate><title>An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging</title><author>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.) ; Tikhonov, A.N ; Hoekstra, F.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-5510daafc1b5ceb3dc6195e3154d93bf46e7828572436f888097a6e0e4776d683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AGING</topic><topic>Aleurone layer</topic><topic>APTITUD PARA LA CONSERVACION</topic><topic>APTITUDE A LA CONSERVATION</topic><topic>Cell membranes</topic><topic>CELL STRUCTURE</topic><topic>ELECTRON PARAMAGNETIC RESONANCE SPECTROS</topic><topic>Embryos</topic><topic>Endosperm</topic><topic>ENVEJECIMIENTO</topic><topic>EPS</topic><topic>ESPECTROSCOPIA ESR</topic><topic>ESR SPECTROSCOPY</topic><topic>ESTRUCTURA CELULAR</topic><topic>FISIOLOGIA POSTCOSECHA</topic><topic>GERMINACION</topic><topic>GERMINATION</topic><topic>GERMOPLASMA</topic><topic>GERMPLASM</topic><topic>KEEPING QUALITY</topic><topic>Laboratorium voor Plantenfysiologie</topic><topic>Laboratory of Plant Physiology</topic><topic>Lipids</topic><topic>LONGEVITE DES SEMENCES</topic><topic>MEASUREMENT</topic><topic>MEDICION</topic><topic>MESURE</topic><topic>PERMEABILIDAD</topic><topic>PERMEABILITE</topic><topic>PERMEABILITY</topic><topic>PHYSIOLOGIE APRES RECOLTE</topic><topic>Plants</topic><topic>PLASMA MEMBRANES</topic><topic>POSTHARVEST PHYSIOLOGY</topic><topic>SEED</topic><topic>SEED LONGEVITY</topic><topic>Seeds</topic><topic>SEMENCE</topic><topic>SEMILLAS</topic><topic>SPECTROSCOPIE RSE</topic><topic>STORAGE LIFE</topic><topic>STRUCTURE CELLULAIRE</topic><topic>TRITICUM</topic><topic>TRITICUM AESTIVUM</topic><topic>VIABILIDAD</topic><topic>VIABILIDAD DE LA SEMILLA</topic><topic>VIABILITE</topic><topic>VIABILITY</topic><topic>VIEILLISSEMENT</topic><topic>Wheat</topic><topic>Whole Plant, Environmental, and Stress Physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.)</creatorcontrib><creatorcontrib>Tikhonov, A.N</creatorcontrib><creatorcontrib>Hoekstra, F.A</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>NARCIS:Publications</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Golovina, E.A. (Russian Academy of Sciences, Moscow, Russia.)</au><au>Tikhonov, A.N</au><au>Hoekstra, F.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>114</volume><issue>1</issue><spage>383</spage><epage>389</epage><pages>383-389</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy and the broadening agent ferricyanide. The line-height ratio between the water and lipid components in the electron paramagnetic resonance spectra of 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (R value) allowed for the quantitative assessment of the plasma membrane permeability in small samples, enabling separate studies of the axis, scutellum, aleurone layer, and starchy endosperm tissue. High R values corresponded to low permeability and vice versa. Starchy endosperm cells had completely permeable plasma membranes even in mature, viable seeds. The loss of germinability with aging coincided with a considerably increased plasma membrane permeability of the embryo axis cells, but not of the scutellum and aleurone layer cells. The threshold R value for the individual axes associated with viability loss was established at 5 to 6, with the total ranging from 0 to more than 12. We suggest that the R value of an individual axis is the result of contributions from all individual cells, each of them characterized by a different permeability. The loss of viability, therefore, corresponds to the accumulation of cells having permeability above a critical level</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>12223711</pmid><doi>10.1104/pp.114.1.383</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1997-05, Vol.114 (1), p.383-389 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_37729 |
| source | JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online |
| subjects | AGING Aleurone layer APTITUD PARA LA CONSERVACION APTITUDE A LA CONSERVATION Cell membranes CELL STRUCTURE ELECTRON PARAMAGNETIC RESONANCE SPECTROS Embryos Endosperm ENVEJECIMIENTO EPS ESPECTROSCOPIA ESR ESR SPECTROSCOPY ESTRUCTURA CELULAR FISIOLOGIA POSTCOSECHA GERMINACION GERMINATION GERMOPLASMA GERMPLASM KEEPING QUALITY Laboratorium voor Plantenfysiologie Laboratory of Plant Physiology Lipids LONGEVITE DES SEMENCES MEASUREMENT MEDICION MESURE PERMEABILIDAD PERMEABILITE PERMEABILITY PHYSIOLOGIE APRES RECOLTE Plants PLASMA MEMBRANES POSTHARVEST PHYSIOLOGY SEED SEED LONGEVITY Seeds SEMENCE SEMILLAS SPECTROSCOPIE RSE STORAGE LIFE STRUCTURE CELLULAIRE TRITICUM TRITICUM AESTIVUM VIABILIDAD VIABILIDAD DE LA SEMILLA VIABILITE VIABILITY VIEILLISSEMENT Wheat Whole Plant, Environmental, and Stress Physiology |
| title | An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging |
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