Potential for analytic breeding in allopolyploids: an illustration from Longiflorum x Asiatic hybrid lilies (Lilium)

• Published in: Euphytica 2009, Vol.166 (3), p.399-409
• Main Authors: Khan, Nadeem, Zhou, Shujun, Ramanna, M. S, Arens, Paul, Herrera, Jeronimo, Visser, Richard G. F, Van Tuyl, Jaap M
• Format: Article
• Language: English
• Subjects: 2n pollen; Agronomy. Soil science and plant productions; allopolyploidy; analytic breeding; aneuploidy; backcrossing; bc2 progenies; Biological and medical sciences; chromosomes; Cytogenetics; diploidy; festuca-pratensis; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; genetic recombination; Genetics and breeding of economic plants; genome; genomic in situ hybridization; gish analysis; heterozygosity; hybrids; intergenomic recombination; interspecific hybridization; interspecific hybrids; introgression; karyotyping; Lilium; Lilium longiflorum; lily hybrids; lolium-multiflorum; nucleic acid hybridization; plant breeding; sexual polyploidization; sweet-potato; triploidy
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-008-9824-0

Ploidy level and intergenomic recombination was studied in interspecific hybrids between Longiflorum x Asiatic lilies (LA hybrid) backcross to Asiatic parents in order to assess the possibility for analytic breeding in lily. By backcrossing the diploid (2n = 2x = 24) F1 interspecific hybrid between Longiflorum x Asiatic lilies to Asiatic parents, 104 BC1 progeny plants were produced. Among these, there were 27 diploids, 73 triploids (2n = 2x = 36) and 4 aneuploids (2x - 1, 2x + 2 or 2x + 3). In addition, by backcrossing triploid BC1 (LAA) plants to diploid Asiatic parents in 2x - 3x and reciprocal combinations, 14 diploid BC2 progenies were produced. Genomic in situ hybridization (GISH) was performed to study the intergenomic recombination and karyotype composition. GISH indicated extensive intergenomic recombination among the chromosomes in LA hybrids. A large number of Longiflorum chromosomes were transmitted to the BC1 progenies from LA hybrids. However, very few Longiflorum chromosomes were transmitted from the BC1 triploid (LAA) plants to the BC2 progenies. The occurrence of diploid plants in the BC progenies of LA hybrids has opened the prospects of analytic breeding in lilies. In this approach, the selection of superior genotypes can be carried out at the diploid level and polyploid forms are synthesized from superior diploid parents. The advantages of analytic breeding are evident: (a) a maximum level of heterozygosity can be attained in the synthetic polyploids and (b) introgression can be achieved with a minimum of linkage drag. Based on GISH results the potential application of analytic breeding in lily allopolyploids has been discussed.