Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds

In order to fully explore the biofunctional potential of proanthocyanidins (PA), isolated and well-characterised PA dimers are of great importance. Current methods to obtain pure A- and B-type dimers are laborious, because they comprise multiple chromatographic steps, often yielding only one or two...

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Bibliographic Details
Published in:Food chemistry 2009-12, Vol.117 (4), p.713-720
Main Authors: Appeldoorn, Maaike M., Sanders, Mark, Vincken, Jean-Paul, Cheynier, Véronique, Le Guernevé, Christine, Hollman, Peter C.H., Gruppen, Harry
Format: Article
Language:English
Subjects:
antioxidant activity
Biological and medical sciences
catechin
cocoa
epicatechin
extract
Food engineering
Food industries
fractionation
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Grape seeds
Isolation
Life Sciences
low-density-lipoprotein
Peanut skins
peanuts
performance liquid-chromatography
polymerization
proanthocyanidins
Procyanidin dimers
procyanidins
Reversed-phase HPLC
separation
yields
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Summary:In order to fully explore the biofunctional potential of proanthocyanidins (PA), isolated and well-characterised PA dimers are of great importance. Current methods to obtain pure A- and B-type dimers are laborious, because they comprise multiple chromatographic steps, often yielding only one or two specific dimers. In the current study, an efficient isolation procedure is described, to isolate a large variety of A-type dimers from peanut skins and B-type dimers from grape seeds. Yields increased 20–400 times for A-type dimers and about 10 times for B-type dimers compared to other methods with a lesser number of chromatographic steps. Dimers isolated from peanut skins were identified as; epicatechin-(2- O-7, 4-8)-catechin (A1), epicatechin-(2- O-7, 4-8)-epicatechin (A2), epicatechin-(2- O-7, 4-6)-catechin, epicatechin-(2- O-7, 4-8)- entcatechin, isolated from peanut skins for the first time, and epicatechin-(4-6)-catechin (B7). Dimers from grape seeds were identified as; epicatechin-(4-8)-catechin (B1), epicatechin-(4-8)-epicatechin (B2), catechin-(4-8)-catechin (B3) and catechin-(4-8)-epicatechin (B4).
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2009.04.047