Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols

• Published in: British journal of nutrition 2009-12, Vol.102 (11), p.1620-1628
• Main Authors: Castagnini, Cinzia, Luceri, Cristina, Toti, Simona, Bigagli, Elisabetta, Caderni, Giovanna, Femia, Angelo P., Giovannelli, Lisa, Lodovici, Maura, Pitozzi, Vanessa, Salvadori, Maddalena, Messerini, Luca, Martin, Rocio, Zoetendal, Erwin G., Gaj, Stan, Eijssen, Lars, Evelo, Chris T., Renard, Catherine M. G. C., Baron, Alain, Dolara, Piero
• Format: Article
• Language: English
• Subjects: 16s ribosomal-rna; animal models; Animals; Apples; assays; Bacteria; Bacteria - isolation & purification; Bacteriodes; Bacteroides fragilis; bacteroides-vulgatus; Biological and medical sciences; bowel-disease; cell proliferation; Colitis - diet therapy; Colitis - genetics; Colitis - microbiology; Colitis - pathology; colon; Cultivars; cyclo-oxygenase-2; Cyclooxygenase 2 - metabolism; diarrhea; Diet; dna-damage; Expression profiles; feces; Feces - microbiology; Feeding. Feeding behavior; Flavonoids - analysis; Food and Nutrition; Fruits; Fundamental and applied biological sciences. Psychology; gastrointestinal-tract; gene expression; Gene Expression Profiling - methods; Gene Expression Regulation; gene-expression profiles; Genetic Predisposition to Disease; gradient gel-electrophoresis; HLA-B27 Antigen - genetics; Humans; Inflammatory bowel disease; Intestinal Mucosa - enzymology; Life Sciences; Male; Malus; Malus - chemistry; Microarray analysis; mucosa; myeloperoxidase; nf-kappa-b; Nitric Oxide Synthase Type II - metabolism; Nutrition research; Nutritional Immunology; Oligonucleotide Array Sequence Analysis - methods; Peroxidase - metabolism; peroxidases; Phenols - analysis; Phytochemicals; polymerase-chain-reaction; Polyphenols; prostaglandin synthase; protective effect; Rats; Rats, Transgenic; Rodents; Species Specificity; Transgenic animals; ulcerative-colitis; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114509990936

Inflammatory bowel diseases (IBD) are immunomediated ailments affecting millions of individuals. Although diet is regarded as an important factor influencing IBD, there are no accepted dietary recommendations presently available. We administered 7·6 % lyophilised apples obtained from two cultivars (Golden Delicious and Marie Ménard, low and high in polyphenols, respectively) to HLA-B27 transgenic rats which develop spontaneous IBD. After 3 months feeding, rats fed Marie Ménard apples had reduced myeloperoxidase activity (3·6 (sem 0·3) v. 2·2 (sem 0·2) U/g tissue; P