Reverse breeding: a novel breeding approach based on engineered meiosis

Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on re...

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Published in:Plant biotechnology journal 2009-12, Vol.7 (9), p.837-845
Main Authors: Dirks, Rob, van Dun, Kees, de Snoo, C. Bastiaan, van den Berg, Mark, Lelivelt, Cilia L.C, Voermans, William, Woudenberg, Leo, de Wit, Jack P.C, Reinink, Kees, Schut, Johan W, van der Zeeuw, Eveline, Vogelaar, Aat, Freymark, Gerald, Gutteling, Evert W, Keppel, Marina N, van Drongelen, Paul, Kieny, Matthieu, Ellul, Philippe, Touraev, Alisher, Ma, Hong, de Jong, Hans, Wijnker, Erik
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
arabidopsis-thaliana
asynapsis
Biological and medical sciences
Biotechnology
Breeding - methods
breeding per chromosome
chromosome segregation
Crops, Agricultural - genetics
Crops, Agricultural - growth & development
Crossing Over, Genetic
cytoplasmic male-sterility
dmc1
engineered meiosis
Fundamental and applied biological sciences. Psychology
gene
Gene Knockdown Techniques
Genetics and breeding of economic plants
Genome, Plant
heterosis
Heterozygote
Meiosis
meiotic prophase-i
plant breeding
Plant breeding: fundamental aspects and methodology
Plant Development
plants
Plants - genetics
protein
recombination
Review article
spore regeneration
univalent segregation
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Summary:Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non-recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity. Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.
ISSN:1467-7644
1467-7652
DOI:10.1111/j.1467-7652.2009.00450.x