Seed transmission of Pepino mosaic virus in tomato

In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment wi...

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Bibliographic Details
Published in:European journal of plant pathology 2010-02, Vol.126 (2), p.145-152
Main Authors: Hanssen, Inge M, Mumford, Rick, Blystad, Dag-Ragnar, Cortez, Isabel, Hasiów-Jaroszewska, Beata, Hristova, Dimitrinka, Pagán, Israel, Pereira, Ana-Maria, Peters, Jeff, Pospieszny, Henryk, Ravnikar, Maja, Stijger, Ineke, Tomassoli, Laura, Varveri, Christina, van der Vlugt, René, Nielsen, Steen Lykke
Format: Article
Language:English
Subjects:
Agriculture
Biological and medical sciences
Biomedical and Life Sciences
Crops
Disease transmission
Ecology
Fundamental and applied biological sciences. Psychology
genomic rna
Genotypes
greenhouse tomatoes
infection
isolate
Life Sciences
Lycopersicon esculentum
number
Original Research
Pathogens
Phytopathology. Animal pests. Plant and forest protection
Plant diseases
Plant Pathology
Plant Sciences
Plant viruses and viroids
population
protein
Seedlings
Seeds
sequence
Tomatoes
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Summary:In this manuscript we provide evidence for the seed transmission of Pepino mosaic virus (PepMV) in tomato. Fruit was harvested from a tomato crop artificially infected with both European and CH2 genotypes of PepMV and more than 100,000 seeds were extracted and cleaned using an enzymatic treatment without disinfection. Infection assays using indicator plants confirmed the presence of viable virus on the seeds. Seeds were distributed to ten different laboratories in three separate batches, where they were germinated and the young plants tested by ELISA. In total over 87,000 plants were tested and 23 positives detected, indicating an overall transmission rate of 0.026%. However, the observed seed transmission rates varied from 0.005% to 0.057%, depending on the seed batch used. Results clearly showed that PepMV can be transmitted from seeds contaminated with virus to seedlings, highlighting the risk of using seeds from PepMV-infected plants and the potential for seed transmission to contribute to the further spread of PepMV.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-009-9528-x