Microanalysis of Plant Cell Wall Polysaccharides

Oligosaccharide Mass Profiling (OLIMP) allows a fast and sensitive assessment of cell wall polymer structure when coupled with Matrix Assisted Laser Desorption Ionisation Time Of Flight Mass Spectrometry (MALDI–TOF MS). The short time required for sample preparation and analysis makes possible the s...

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Bibliographic Details
Published in:Molecular plant 2009-09, Vol.2 (5), p.922-932
Main Authors: Obel, Nicolai, Erben, Veronika, Schwarz, Tatjana, Kühnel, Stefan, Fodor, Andrea, Pauly, Markus
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - chemistry
Arabidopsis - metabolism
arabidopsis-thaliana
architecture
biosynthesis
carbohydrate gel-electrophoresis
Carbohydrate Metabolism
cell expansion
Cell Wall - chemistry
Cell Wall - metabolism
cell walls
Dissection
genes
Glucans - chemistry
Glucans - metabolism
identification
laser microdissection
laser-capture microdissection
mass spectrometry
oligosaccharides
Principal Component Analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Xylans - chemistry
Xylans - metabolism
xyloglucan
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Summary:Oligosaccharide Mass Profiling (OLIMP) allows a fast and sensitive assessment of cell wall polymer structure when coupled with Matrix Assisted Laser Desorption Ionisation Time Of Flight Mass Spectrometry (MALDI–TOF MS). The short time required for sample preparation and analysis makes possible the study of a wide range of plant organs, revealing a high degree of heterogeneity in the substitution pattern of wall polymers such as the cross-linking glycan xyloglucan and the pectic polysaccharide homogalacturonan. The high sensitivity of MALDI–TOF allows the use of small amounts of samples, thus making it possible to investigate the wall structure of single cell types when material is collected by such methods as laser micro-dissection. As an example, the analysis of the xyloglucan structure in the leaf cell types outer epidermis layer, entire epidermis cell layer, palisade mesophyll cells, and vascular bundles were investigated. OLIMP is amenable to in situ wall analysis, where wall polymers are analyzed on unprepared plant tissue itself without first isolating cell walls. In addition, OLIMP enables analysis of wall polymers in Golgi-enriched fractions, the location of nascent matrix polysaccharide biosynthesis, enabling separation of the processes of wall biosynthesis versus post-deposition apoplastic metabolism. These new tools will make possible a semi-quantitative analysis of the cell wall at an unprecedented level.
ISSN:1674-2052
1752-9867
DOI:10.1093/mp/ssp046