High-performance liquid chromatographic method for the analysis of anthraquinone glycosides and aglycones in madder root ( Rubia tinctorum L.)

A HPLC method has been developed for the simultaneous characterisation of anthraquinone glycosides and aglycones in extracts of Rubia tinctorum L. The anthraquinones are separated on an end-capped C 18-RP column with a water–acetonitrile gradient as eluent and measured with UV detection at 250 nm. W...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-08, Vol.816 (2), p.277-281
Main Authors: Derksen, Goverdina C.H., van Beek, Teris A., de Groot, Æde, Capelle, Anthony
Format: Article
Language:English
Subjects:
Alizarin
Analytical, structural and metabolic biochemistry
Anthraquinones
Aromatic and heterocyclic compounds
Biological and medical sciences
EPS
Fundamental and applied biological sciences. Psychology
Heterocyclic compounds, pigments
Laboratorium voor Organische chemie
Laboratory for Organic Chemistry
Lucidin
Lucidin primeveroside
Organic Chemistry
Organische Chemie
Other biological molecules
Purpurin
Quinizarin
Ruberythric acid
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Summary:A HPLC method has been developed for the simultaneous characterisation of anthraquinone glycosides and aglycones in extracts of Rubia tinctorum L. The anthraquinones are separated on an end-capped C 18-RP column with a water–acetonitrile gradient as eluent and measured with UV detection at 250 nm. With this method the glycosides lucidin primeveroside and ruberythric acid and the aglycones lucidin, alizarin, purpurin, quinizarin and 2,6-dihydroxyanthraquinone can be analysed. Lucidin which is not commercially available was synthesised starting from resorcinol and phthalic anhydride. The glycosides ruberythric acid and lucidin primeveroside are commercially available as a mixture and were separated by droplet counter-current chromatography in ascending flow with chloroform–methanol–water as eluents prior to their use as standards.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00492-0