A potato pathogenesis-related protein gene, StPRp27, contributes to race-nonspecific resistance against Phytophthora infestans

• Published in: Molecular biology reports 2012-02, Vol.39 (2), p.1909-1916
• Main Authors: Shi, Xiaolei, Tian, Zhendong, Liu, Jun, van der Vossen, Edwin A. G., Xie, Conghua
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Breeding; Breeding - methods; broad-spectrum resistance; Disease resistance; Disease Resistance - genetics; DNA Primers - genetics; Evolution; expression; Gene Silencing; Histology; identification; infection; Inoculation; Late blight; late blight resistance; Leaves; Life Sciences; Molecular biology; Molecular modelling; Morphology; Nicotiana; Nicotiana benthamiana; Overexpression; Pathogenesis; Pathogenesis-related proteins; Pathogens; Phytophthora; Phytophthora infestans; Plant diseases; Plant Diseases - microbiology; Plant Proteins - genetics; population; Potatoes; Proteins; quantitative resistance; r-gene; Races; Real-Time Polymerase Chain Reaction; Solanum tuberosum; solanum-bulbocastanum; tobacco
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-0937-5

Late blight caused by Phytophthora infestans is the most important disease of potato. Many efforts have been made to understand molecular mechanism of the durable resistance to address the challenge raised by rapid evolution of the pathogen. A pathogenesis related protein (PR) gene StPRp27 was previously isolated from the potato leaves challenged by P. infestans . The sequence analysis and expression pattern reveal that StPRp27 may be associated with resistance to P. infestans . In present research, transient expression of StPRp27 in Nicotiana benthamiana enhanced resistance to P. infestans isolates 99189 and PY23 indicating its potential contribution to the disease resistance. These findings were also confirmed by over-expression of StPRp27 in potato cv. E-potato 3, which significantly slowed down the development of the disease after inoculation with a mixture of P . infestans races. Further, silencing of StPRp27 homologous genes in N. benthamiana harboring dominant Phytophthora resistance gene Rpi-blb1 or Rpi-blb2 showed no effects on the resistance triggered by these R genes. Our results suggest that StPRp27 contributes to a race-nonspecific resistance against P. infestans by inhibiting the disease development and has a potential use in selection and breeding for durable resistance to late blight.