Early life developmental effects of marine persistent organic pollutants on the sea urchin Psammechinus miliaris

• Published in: Ecotoxicology and environmental safety 2011-11, Vol.74 (8), p.2182-2192
• Main Authors: Anselmo, Henrique M.R., Koerting, Lina, Devito, Sarah, van den Berg, Johannes H.J., Dubbeldam, Marco, Kwadijk, Christiaan, Murk, Albertinka J.
• Format: Article
• Language: English
• Subjects: abnormal development; Abnormalities; acids; adverse effects; amphibians; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Applied ecology; aquatic toxicity; Bioassay; bioassays; Biological and medical sciences; early development; echinodermata; Echinoid; Echinoida; Echinoidea; Ecotoxicology, biological effects of pollution; ELS: early life stage; embryogenesis; Embryonic Development - drug effects; embryos; exposure; Fertilization - drug effects; fish; Fundamental and applied biological sciences. Psychology; General aspects; HBCD; Hydrocarbons, Brominated - toxicity; Larva - drug effects; Larva - growth & development; larvae; larval development; Marine; Morphological abnormalities; mortality; north-sea; Organic Chemicals - toxicity; paracentrotus-lividus; PCB 126; pcbs; persistent organic pollutants; PFOS; Pollutants; Polybrominated Biphenyls - toxicity; polychlorinated-biphenyls; Psammechinus miliaris; Sea urchins; Sea Urchins - drug effects; Sea Urchins - growth & development; Sea water ecosystems; seawater; Seawater - chemistry; sediment; Synecology; TBBPA; TCDD; TCS; Toxic; Toxicity; Toxicology; Triclosan - toxicity; Water Pollutants, Chemical - toxicity; western scheldt estuary
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2011.07.037

A new 16-day echinoid early life stage (ELS) bioassay was developed to allow for prolonged observation of possible adverse effects during embryogenesis and larval development of the sea urchin Psammechinus miliaris. Subsequently, the newly developed bioassay was applied to study the effects of key marine persistent organic pollutants (POPs). Mortality, morphological abnormalities and larval development stages were quantified at specific time points during the 16-day experimental period. In contrast to amphibians and fish, P. miliaris early life development was not sensitive to dioxin-like toxicity in the prolonged early life stage test. Triclosan (TCS) levels higher than 500 nM were acutely toxic during embryo development. Morphological abnormalities were induced at concentrations higher than 50 nM hexabromocyclododecane (HBCD) and 1000 nM tetrabromobisphenol A (TBBPA). Larval development was delayed above 25 nM HBCD and 500 nM TBBPA. Heptadecafluorooctane sulfonic acid (PFOS) exposure slightly accelerated larval development at 9 days post-fertilization (dpf). However, the accelerated development was no longer observed at the end of the test period (16 dpf). The newly developed 16-day echinoid ELS bioassay proved to be sensitive to toxic effects of POPs that can be monitored for individual echinoid larvae. The most sensitive and dose related endpoint was the number of developmental penalty points. By manipulation of the housing conditions, the reproductive season could be extended from 3 to 9 months per year and the ELS experiments could be performed in artificial sea water as well. ► A 16-day echinoid ELS bioassay was developed to assess toxic effects of POPs on larval development. ► Prolonged ELS bioassay was sensitive to sub-lethal effects of POPs, with HBCD being the most toxic. ► Developmental penalty points were the most sensitive endpoint. ► Echinoid ELS bioassay could be applied for environmental risk assessment of POPs on marine ecosystems. ► Use of echinoids could potentially contribute to the reduction of vertebrate toxicity studies.