Infection of Alstroemeria Plants with Tomato yellow ring virus in Iran

Alstroemeria cv. Ovation plants with virus‐like necrotic spots and streaks on leaves and petals were observed in greenhouses in Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces, Iran. Samples with virus‐like symptoms reacted positively in enzyme‐linked immunosorbent assay with a polyclonal...

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Bibliographic Details
Published in:Journal of phytopathology 2012-01, Vol.160 (1), p.45-47
Main Authors: Beikzadeh, Nasser, Bayat, Hossein, Jafarpour, Behrooz, Rohani, Hamid, Peters, Dick, Hassani-Mehraban, Afshin
Format: Article
Language:English
Subjects:
Alstroemeria
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Iran
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Tomato yellow ring virus
tospovirus
virus strains
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Summary:Alstroemeria cv. Ovation plants with virus‐like necrotic spots and streaks on leaves and petals were observed in greenhouses in Khorasan Razavi (Mashhad) and Markazi (Mahallat) provinces, Iran. Samples with virus‐like symptoms reacted positively in enzyme‐linked immunosorbent assay with a polyclonal antibody raised against Tomato yellow ring virus (TYRV) nucleocapsid (N) protein. TYRV‐specific primers were used in a reverse transcription‐polymerase chain reaction to amplify the N gene. The deduced amino acid sequences of the obtained amplicon revealed 99% identity to the N protein of an isolate of TYRV isolated from tomato (TYRV‐t).
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2011.01851.x