Gut bacteria–host metabolic interplay during conventionalisation of the mouse germfree colon

• Published in: The ISME Journal 2013-04, Vol.7 (4), p.743-755
• Main Authors: El Aidy, Sahar, Derrien, Muriel, Merrifield, Claire A, Levenez, Florence, Doré, Joël, Boekschoten, Mark V, Dekker, Jan, Holmes, Elaine, Zoetendal, Erwin G, van Baarlen, Peter, Claus, Sandrine P, Kleerebezem, Michiel
• Format: Article
• Language: English
• Subjects: 631/208/212/2019; 631/326/41/2142; 692/698/2741/2135; Agricultural sciences; Alanine; Animals; Bacilli; Bacteria - classification; Bacteria - metabolism; Biomedical and Life Sciences; Colon; Colon - metabolism; Colon - microbiology; Colonization; colorectal-cancer; Diet; Digestive tract; Ecology; Energy metabolism; Evolutionary Biology; Gastrointestinal tract; Gene expression; Gene Expression Profiling; Germ-Free Life; Germfree; Glutamine; Glycine; human intestine; immune-system; Intestinal Mucosa - microbiology; Life Sciences; Male; Mammals; Metabolites; Metabolomics; Metagenome; mice; Mice - microbiology; Mice, Inbred C57BL; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; microbiota; Mucosa; nmr; Nutrients; Original; original-article; polysaccharide utilization; Proteobacteria; Random Allocation; selenomonas-ruminantium; Symbiosis; Urine; waste-water
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2012.142

The interplay between dietary nutrients, gut microbiota and mammalian host tissues of the gastrointestinal tract is recognised as highly relevant for host health. Combined transcriptome, metabonome and microbial profiling tools were employed to analyse the dynamic responses of germfree mouse colonic mucosa to colonisation by normal mouse microbiota (conventionalisation) at different time-points during 16 days. The colonising microbiota showed a shift from early (days 1 and 2) to later colonisers (days 8 and 16). The dynamic changes in the microbial community were rapidly reflected by the urine metabolic profiles (day 1) and at later stages (day 4 onward) by the colon mucosa transcriptome and metabolic profiles. Correlations of host transcriptomes, metabolite patterns and microbiota composition revealed associations between Bacilli and Proteobacteria, and differential expression of host genes involved in energy and anabolic metabolism. Differential gene expression correlated with scyllo - and myo -inositol, glutamine, glycine and alanine levels in colonic tissues during the time span of conventionalisation. Our combined time-resolved analyses may help to expand the understanding of host–microbe molecular interactions during the microbial establishment.