Promoting chain elongation in mixed culture acidification reactors by addition of ethanol

• Published in: Biomass & bioenergy 2013-01, Vol.48, p.10-16
• Main Authors: Grootscholten, T.I.M., Kinsky dal Borgo, F., Hamelers, H.V.M., Buisman, C.J.N.
• Format: Article
• Language: English
• Subjects: Acidification; Alternative fuels. Production and utilization; Applied sciences; Biofuel production; Biological and medical sciences; biomass; Biotechnology; Carboxylate; cellulose; clostridium-kluyveri; digestion; Energy; Exact sciences and technology; fatty-acids; Fuels; Fundamental and applied biological sciences. Psychology; hydrolysis; Industrial applications and implications. Economical aspects; liquid fuels; MCFA; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Miscellaneous; Mixed culture fermentation; OFMSW; solid-waste; VFA; Waste
• ISSN: 0961-9534; 1873-2909
• DOI: 10.1016/j.biombioe.2012.11.019

In this research we investigate a microbial production process to produce medium chain fatty acids (MCFAs) based on the organic fraction of municipal solid waste (OFMSW). In this microbial production process, called chain elongation, bacteria produce medium chain fatty acids (MCFAs) from ethanol and volatile fatty acids (VFAs). MCFAs could be used as new biomass based building blocks for the chemical and fuel industry. The objective of this article is to investigate whether chain elongation can be promoted during acidification of OFMSW by addition of ethanol. The results show that chain elongation can be promoted during acidification of OFMSW by addition of ethanol. However, the hydrolysis rate and the carboxylic acid yield of the OFMSW in reactors with ethanol additions were lower than the hydrolysis rate and the carboxylic acid yield than in reactors without ethanol additions. Further research is required to determine whether a combined chain elongation and acidification reactor or a separated reactor system is more advantageous for MCFA production from OFMSW. ► Production of medium chain fatty acids from municipal solid waste and ethanol. ► Insight in production of caproate and consumption of in-situ produced ethanol. ► Ethanol additions reduced propionate, butyrate and valerate concentrations. ► Ethanol additions hardly reduced acetate concentrations. ► Hydrolysis rate was lower in experiments with ethanol additions.