Biomass Estimation of Coniothyrium Minitans in Solid-State Fermentation

For the estimation of the amount of biomass in solid-state fermentation, various indirect methods are available. The suitability of respiration measurements and the content of various cell components were evaluated for Coniothyrium minitans grown on different media. Protein and nucleic acid measurem...

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Bibliographic Details
Published in:Enzyme and microbial technology 1998-05, Vol.22 (6), p.480-486
Main Authors: Ooijkaas, L.P., Tramper, J., Buitelaar, R.M.
Format: Article
Language:English
Subjects:
Agrotechnological Research Institute
Amines
Biological and medical sciences
Biomass
Biomass estimation
Biotechnology
conidiation
Coniothyrium minitans
Dextrose
Fundamental and applied biological sciences. Psychology
Fungi
growth
Instituut voor Agrotechnologisch Onderzoek
methods
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Nucleic acids
Plant cell culture
Proteins
Sectie Proceskunde
solid-state fermentation
Sub-department of Food and Bioprocess Engineering
VLAG
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Summary:For the estimation of the amount of biomass in solid-state fermentation, various indirect methods are available. The suitability of respiration measurements and the content of various cell components were evaluated for Coniothyrium minitans grown on different media. Protein and nucleic acid measurements are useful as indicators for total biomass when C. minitans is grown on potato dextrose agar (PDA) but not on nutrient agar with glucose (NAG) or starch (NAS) as a carbon source. None of the methods tested are reliable enough to discriminate between the amounts of mycelial and conidial biomass of C. minitans when used solely; however, using PDA as growth medium, a distinction can be made between mycelial and conidial biomass by combining the content of glucosamine, protein, and nucleic acid with each other or with spore counts.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(97)00246-9