A bead-based suspension array for the serological detection of Trichinella in pigs

The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory–secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine...

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Bibliographic Details
Published in:The veterinary journal (1997) 2013-06, Vol.196 (3), p.439-444
Main Authors: van der Wal, F.J., Achterberg, R.P., Kant, A., Maassen, C.B.M.
Format: Article
Language:English
Subjects:
Animals
antibodies
antigens
blood serum
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
farms
Human
humans
immunoassay
Indexing in process
linked-immunosorbent-assay
multiplexed luminex assay
nonstructural proteins
Pig
Sensitivity and Specificity
serodiagnosis
Serologic Tests - veterinary
Serology
spiralis
Suspension array
Swine
Swine Diseases - blood
Swine Diseases - diagnosis
Swine Diseases - parasitology
Trichinella - isolation & purification
Trichinella spiralis
Trichinellosis - blood
Trichinellosis - diagnosis
Trichinellosis - parasitology
Trichinellosis - veterinary
trichinosis
virus
Zoonosis
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Summary:The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory–secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine antibody. The assay was evaluated by testing pig sera from farms where trichinellosis was endemic and comparing the results with those obtained using two commercially available ELISAs. With cut-offs established by receiver operating characteristic (ROC) analysis, digestion-negative sera from a Trichinella-free population of pigs were deemed seronegative. When anti-swine antibody was replaced with protein A/G, higher test sensitivity (94% vs. 88%) at similar test specificity (95%), was achieved. The potential use of this assay in species other than swine was also demonstrated by testing human sera.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2012.10.029