A bead-based suspension array for the serological detection of Trichinella in pigs

• Published in: The veterinary journal (1997) 2013-06, Vol.196 (3), p.439-444
• Main Authors: van der Wal, F.J., Achterberg, R.P., Kant, A., Maassen, C.B.M.
• Format: Article
• Language: English
• Subjects: Animals; antibodies; antigens; blood serum; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; farms; Human; humans; immunoassay; Indexing in process; linked-immunosorbent-assay; multiplexed luminex assay; nonstructural proteins; Pig; Sensitivity and Specificity; serodiagnosis; Serologic Tests - veterinary; Serology; spiralis; Suspension array; Swine; Swine Diseases - blood; Swine Diseases - diagnosis; Swine Diseases - parasitology; Trichinella - isolation & purification; Trichinella spiralis; Trichinellosis - blood; Trichinellosis - diagnosis; Trichinellosis - parasitology; Trichinellosis - veterinary; trichinosis; virus; Zoonosis
• ISSN: 1090-0233; 1532-2971
• DOI: 10.1016/j.tvjl.2012.10.029

The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory–secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine antibody. The assay was evaluated by testing pig sera from farms where trichinellosis was endemic and comparing the results with those obtained using two commercially available ELISAs. With cut-offs established by receiver operating characteristic (ROC) analysis, digestion-negative sera from a Trichinella-free population of pigs were deemed seronegative. When anti-swine antibody was replaced with protein A/G, higher test sensitivity (94% vs. 88%) at similar test specificity (95%), was achieved. The potential use of this assay in species other than swine was also demonstrated by testing human sera.