FRET-FLIM applications in plant systems

A hallmark of cellular processes is the spatio-temporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination...

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Bibliographic Details
Published in:Protoplasma 2014-03, Vol.251 (2), p.383-394
Main Authors: Bücherl, Christoph A., Bader, Arjen, Westphal, Adrie H., Laptenok, Sergey P., Borst, Jan Willem
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
fluorescence correlation spectroscopy
Fluorescence Resonance Energy Transfer - methods
fusion-proteins
global analysis
Green Fluorescent Proteins - chemistry
Life Sciences
lifetime imaging microscopy
living cells
Microscopy, Fluorescence - methods
molecular-interactions
Optical Imaging
Plant Cells - chemistry
Plant Cells - ultrastructure
Plant Sciences
Plants - chemistry
Plants - ultrastructure
protein-protein interactions
resonance energy-transfer
spatial-resolution
Special Issue: New/Emerging Techniques in Biological Microscopy
subcellular resolution
Zoology
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Summary:A hallmark of cellular processes is the spatio-temporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination with advances in fluorescence microscopy have revolutionised this field of research by providing the genetic tools to investigate the spatio-temporal dynamics of biomolecules in live cells. In particular, fluorescence lifetime imaging microscopy (FLIM) has become an inevitable technique for spatially resolving cellular processes and physical interactions of cellular components in real time based on the detection of Förster resonance energy transfer (FRET). In this review, we provide a theoretical background of FLIM as well as FRET-FLIM analysis. Furthermore, we show two cases in which advanced microscopy applications revealed many new insights of cellular processes in living plant cells as well as in whole plants.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-013-0595-7