Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

• Published in: Theoretical and applied genetics 2014-06, Vol.127 (6), p.1353-1364
• Main Authors: Zhang, Chunzhi, Liu, Lei, Wang, Xiaoxuan, Vossen, Jack, Li, Guangcun, Li, Tao, Zheng, Zheng, Gao, Jianchang, Guo, Yanmei, Visser, Richard G. F, Li, Junming, Bai, Yuling, Du, Yongchen
• Format: Article
• Language: English
• Subjects: Agricultural research; Agriculture; Amino Acid Sequence; avirulence genes; bacterial artificial chromosomes; Base Sequence; Biochemistry; Biomedical and Life Sciences; Biotechnology; breeding; Chromosome Mapping; Chromosomes; clonal lineage; Cloning; cultivars; cultivated potato; disease resistance; Disease Resistance - genetics; Diseases and pests; Epidemics; evolution; Fungicides; Gene expression; Genes; Genes, Plant; Genetic aspects; Genetic research; Health aspects; late blight resistance; Life Sciences; Lycopersicon esculentum; Molecular Sequence Data; Original Paper; Pathogens; phenotype; Phytophthora infestans; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - physiology; Plant-pathogen relationships; Polymorphism, Single Nucleotide; potato late blight; potatoes; powdery mildew resistance; Proteins; Quantitative Trait Loci; r-gene; Sequence Alignment; Solanum; Solanum - genetics; Solanum pimpinellifolium; Solanum tuberosum; tomato hybrid; tomatoes; Water molds
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-014-2303-1

KEY MESSAGE : Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein. Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).