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Distribution of anticoagulant rodenticide resistance in Rattus norvegicus in the Netherlands according to Vkorc1 mutations
BACKGROUND: Rodenticide resistance to anticoagulants in Rattus norvegicus will lead to increased difficulties in combating these pest animals. Here, the authors present the results of a survey in the Netherlands where tissue samples and droppings were tested using a newly developed TaqMan PCR test f...
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Published in: | Pest management science 2014-11, Vol.70 (11), p.1761-1766 |
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description | BACKGROUND: Rodenticide resistance to anticoagulants in Rattus norvegicus will lead to increased difficulties in combating these pest animals. Here, the authors present the results of a survey in the Netherlands where tissue samples and droppings were tested using a newly developed TaqMan PCR test for genotypic variation at codon 139 in the Vkorc1 gene associated with anticoagulant rodenticide resistance. Test results are linked to results of a questionnaire that was conducted among pest controllers. RESULTS: Genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus can be encountered in many parts of the Netherlands. In 34/61 rat tails, a genotype was found that is linked to anticoagulant rodenticide resistance (56%). In droppings, 42/169 samples (25%) showed a resistance‐mediating genotype. In addition, indications of a clear genetic substructure in the Netherlands were found. In some regions, only resistance‐mediating genotypes were found, corroborating results from the questionnaire in which pest controllers indicated they suspected resistance to anticoagulant rodenticides. CONCLUSION: This is the first study to demonstrate the presence of multiple genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus in the Netherlands. As rodenticides should keep their efficacy because they are a last resort in rodent management, more studies are urgently needed that link specific genetic mutations to the efficacy of active substances. © 2014 Society of Chemical Industry |
doi_str_mv | 10.1002/ps.3809 |
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Here, the authors present the results of a survey in the Netherlands where tissue samples and droppings were tested using a newly developed TaqMan PCR test for genotypic variation at codon 139 in the Vkorc1 gene associated with anticoagulant rodenticide resistance. Test results are linked to results of a questionnaire that was conducted among pest controllers. RESULTS: Genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus can be encountered in many parts of the Netherlands. In 34/61 rat tails, a genotype was found that is linked to anticoagulant rodenticide resistance (56%). In droppings, 42/169 samples (25%) showed a resistance‐mediating genotype. In addition, indications of a clear genetic substructure in the Netherlands were found. In some regions, only resistance‐mediating genotypes were found, corroborating results from the questionnaire in which pest controllers indicated they suspected resistance to anticoagulant rodenticides. CONCLUSION: This is the first study to demonstrate the presence of multiple genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus in the Netherlands. As rodenticides should keep their efficacy because they are a last resort in rodent management, more studies are urgently needed that link specific genetic mutations to the efficacy of active substances. © 2014 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.3809</identifier><identifier>PMID: 24753361</identifier><identifier>CODEN: PMSCFC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Anticoagulants ; Anticoagulants - pharmacology ; anticoagulants, integrated pest management ; berk ; Biological and medical sciences ; chlorophacinone ; Control ; controllers ; Drug Resistance - genetics ; Feces ; Fundamental and applied biological sciences. Psychology ; gene ; genes ; genetic mutation ; Genetic Variation ; genotype ; Genotype & phenotype ; germany ; integrated pest management ; Mutation ; Netherlands ; norway rats ; Pest control ; Phytopathology. Animal pests. Plant and forest protection ; Polymerase chain reaction ; Protozoa. Invertebrates ; questionnaires ; rat ; rats ; Rats - genetics ; Rattus norvegicus ; Real-Time Polymerase Chain Reaction ; Resistance to control ; rodent control ; Rodent Control - methods ; rodenticide resistance ; rodenticides ; Rodenticides - pharmacology ; Rodents ; surveys ; Surveys and Questionnaires ; tail ; tests ; tyrosine139cysteine focus ; vertebrate pests ; Vitamin K Epoxide Reductases - genetics ; warfarin ; westphalia</subject><ispartof>Pest management science, 2014-11, Vol.70 (11), p.1761-1766</ispartof><rights>2014 The Authors. published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry</rights><rights>2015 INIST-CNRS</rights><rights>2014 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</rights><rights>Copyright Wiley Subscription Services, Inc. Nov 2014</rights><rights>2014 The Authors. published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry 2014</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5769-5b380bec29bb72a80cb24add94b4713d8f80e4abaa7f0d4b854f2150a909b95b3</citedby><cites>FETCH-LOGICAL-c5769-5b380bec29bb72a80cb24add94b4713d8f80e4abaa7f0d4b854f2150a909b95b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28850793$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24753361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meerburg, Bastiaan G</creatorcontrib><creatorcontrib>van Gent‐Pelzer, Marga PE</creatorcontrib><creatorcontrib>Schoelitsz, Bruce</creatorcontrib><creatorcontrib>van der Lee, Theo AJ</creatorcontrib><title>Distribution of anticoagulant rodenticide resistance in Rattus norvegicus in the Netherlands according to Vkorc1 mutations</title><title>Pest management science</title><addtitle>Pest. Manag. Sci</addtitle><description>BACKGROUND: Rodenticide resistance to anticoagulants in Rattus norvegicus will lead to increased difficulties in combating these pest animals. Here, the authors present the results of a survey in the Netherlands where tissue samples and droppings were tested using a newly developed TaqMan PCR test for genotypic variation at codon 139 in the Vkorc1 gene associated with anticoagulant rodenticide resistance. Test results are linked to results of a questionnaire that was conducted among pest controllers. RESULTS: Genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus can be encountered in many parts of the Netherlands. In 34/61 rat tails, a genotype was found that is linked to anticoagulant rodenticide resistance (56%). In droppings, 42/169 samples (25%) showed a resistance‐mediating genotype. In addition, indications of a clear genetic substructure in the Netherlands were found. In some regions, only resistance‐mediating genotypes were found, corroborating results from the questionnaire in which pest controllers indicated they suspected resistance to anticoagulant rodenticides. CONCLUSION: This is the first study to demonstrate the presence of multiple genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus in the Netherlands. As rodenticides should keep their efficacy because they are a last resort in rodent management, more studies are urgently needed that link specific genetic mutations to the efficacy of active substances. © 2014 Society of Chemical Industry</description><subject>Animals</subject><subject>Anticoagulants</subject><subject>Anticoagulants - pharmacology</subject><subject>anticoagulants, integrated pest management</subject><subject>berk</subject><subject>Biological and medical sciences</subject><subject>chlorophacinone</subject><subject>Control</subject><subject>controllers</subject><subject>Drug Resistance - genetics</subject><subject>Feces</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>genes</subject><subject>genetic mutation</subject><subject>Genetic Variation</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>germany</subject><subject>integrated pest management</subject><subject>Mutation</subject><subject>Netherlands</subject><subject>norway rats</subject><subject>Pest control</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Polymerase chain reaction</subject><subject>Protozoa. Invertebrates</subject><subject>questionnaires</subject><subject>rat</subject><subject>rats</subject><subject>Rats - genetics</subject><subject>Rattus norvegicus</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Resistance to control</subject><subject>rodent control</subject><subject>Rodent Control - methods</subject><subject>rodenticide resistance</subject><subject>rodenticides</subject><subject>Rodenticides - pharmacology</subject><subject>Rodents</subject><subject>surveys</subject><subject>Surveys and Questionnaires</subject><subject>tail</subject><subject>tests</subject><subject>tyrosine139cysteine focus</subject><subject>vertebrate pests</subject><subject>Vitamin K Epoxide Reductases - genetics</subject><subject>warfarin</subject><subject>westphalia</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1km1rFDEQxxdRbK3iN9BAEQW5mk2yD_GFID2tQqnas3rvwiSbvabdS85kt7V-eme981DBN8kw-c3MfyaTZQ9zepBTyl6s0gGvqbyV7eYFKydCyvr21q7nO9m9lC4opVJKdjfbYaIqOC_z3ezH1KU-Oj30LngSWgK-dybAYujQIjE0dnS4xpJoE7LgjSXOk1Po-yERH-KVXTiDJjr7c0tOLJ4Ro5tEwJgQG-cXpA_ky2WIJifLoYexWLqf3WmhS_bB5t7Lzt6--Xz4bnL84ej94evjiSmqUk4KjZ1pa5jUumJQU6OZgKaRQosq503d1tQK0ABVSxuh60K0LC8oSCq1xOi97OU67zUsrEcx1isP0bikAjjVOR0h3qjrISrfjddq0EmJoixYhcGv1sHoXNrG4DQidGoV3XIMGhP8_eLduVqEKyUYr2teYoJnmwQxfBts6tXSJWM7HJANQ1J5WfKScfEL3f8HvQhD9DgbpLCjUqIkpJ6uKRNDStG2WzE5VeMyqFVS4zIg-ehP7Vvu9-8j8GQDQDLQtRE_F6ey5eq6oJXkyD3fTNB19uZ_9dTH2absZE3jttjvWxripSorXhXq68mRkrP5_HQ6_aQo8o_XfAtBwSKigrMZo9gwpTkvBOc_AbJn50o</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Meerburg, Bastiaan G</creator><creator>van Gent‐Pelzer, Marga PE</creator><creator>Schoelitsz, Bruce</creator><creator>van der Lee, Theo AJ</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>QVL</scope></search><sort><creationdate>201411</creationdate><title>Distribution of anticoagulant rodenticide resistance in Rattus norvegicus in the Netherlands according to Vkorc1 mutations</title><author>Meerburg, Bastiaan G ; van Gent‐Pelzer, Marga PE ; Schoelitsz, Bruce ; van der Lee, Theo AJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5769-5b380bec29bb72a80cb24add94b4713d8f80e4abaa7f0d4b854f2150a909b95b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anticoagulants</topic><topic>Anticoagulants - pharmacology</topic><topic>anticoagulants, integrated pest management</topic><topic>berk</topic><topic>Biological and medical sciences</topic><topic>chlorophacinone</topic><topic>Control</topic><topic>controllers</topic><topic>Drug Resistance - genetics</topic><topic>Feces</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>genes</topic><topic>genetic mutation</topic><topic>Genetic Variation</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>germany</topic><topic>integrated pest management</topic><topic>Mutation</topic><topic>Netherlands</topic><topic>norway rats</topic><topic>Pest control</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Polymerase chain reaction</topic><topic>Protozoa. Invertebrates</topic><topic>questionnaires</topic><topic>rat</topic><topic>rats</topic><topic>Rats - genetics</topic><topic>Rattus norvegicus</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Resistance to control</topic><topic>rodent control</topic><topic>Rodent Control - methods</topic><topic>rodenticide resistance</topic><topic>rodenticides</topic><topic>Rodenticides - pharmacology</topic><topic>Rodents</topic><topic>surveys</topic><topic>Surveys and Questionnaires</topic><topic>tail</topic><topic>tests</topic><topic>tyrosine139cysteine focus</topic><topic>vertebrate pests</topic><topic>Vitamin K Epoxide Reductases - genetics</topic><topic>warfarin</topic><topic>westphalia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meerburg, Bastiaan G</creatorcontrib><creatorcontrib>van Gent‐Pelzer, Marga PE</creatorcontrib><creatorcontrib>Schoelitsz, Bruce</creatorcontrib><creatorcontrib>van der Lee, Theo AJ</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>NARCIS:Publications</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meerburg, Bastiaan G</au><au>van Gent‐Pelzer, Marga PE</au><au>Schoelitsz, Bruce</au><au>van der Lee, Theo AJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of anticoagulant rodenticide resistance in Rattus norvegicus in the Netherlands according to Vkorc1 mutations</atitle><jtitle>Pest management science</jtitle><addtitle>Pest. Manag. Sci</addtitle><date>2014-11</date><risdate>2014</risdate><volume>70</volume><issue>11</issue><spage>1761</spage><epage>1766</epage><pages>1761-1766</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><coden>PMSCFC</coden><abstract>BACKGROUND: Rodenticide resistance to anticoagulants in Rattus norvegicus will lead to increased difficulties in combating these pest animals. Here, the authors present the results of a survey in the Netherlands where tissue samples and droppings were tested using a newly developed TaqMan PCR test for genotypic variation at codon 139 in the Vkorc1 gene associated with anticoagulant rodenticide resistance. Test results are linked to results of a questionnaire that was conducted among pest controllers. RESULTS: Genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus can be encountered in many parts of the Netherlands. In 34/61 rat tails, a genotype was found that is linked to anticoagulant rodenticide resistance (56%). In droppings, 42/169 samples (25%) showed a resistance‐mediating genotype. In addition, indications of a clear genetic substructure in the Netherlands were found. In some regions, only resistance‐mediating genotypes were found, corroborating results from the questionnaire in which pest controllers indicated they suspected resistance to anticoagulant rodenticides. CONCLUSION: This is the first study to demonstrate the presence of multiple genetic mutations at codon 139 of the Vkorc1 gene in R. norvegicus in the Netherlands. As rodenticides should keep their efficacy because they are a last resort in rodent management, more studies are urgently needed that link specific genetic mutations to the efficacy of active substances. © 2014 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>24753361</pmid><doi>10.1002/ps.3809</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Anticoagulants Anticoagulants - pharmacology anticoagulants, integrated pest management berk Biological and medical sciences chlorophacinone Control controllers Drug Resistance - genetics Feces Fundamental and applied biological sciences. Psychology gene genes genetic mutation Genetic Variation genotype Genotype & phenotype germany integrated pest management Mutation Netherlands norway rats Pest control Phytopathology. Animal pests. Plant and forest protection Polymerase chain reaction Protozoa. Invertebrates questionnaires rat rats Rats - genetics Rattus norvegicus Real-Time Polymerase Chain Reaction Resistance to control rodent control Rodent Control - methods rodenticide resistance rodenticides Rodenticides - pharmacology Rodents surveys Surveys and Questionnaires tail tests tyrosine139cysteine focus vertebrate pests Vitamin K Epoxide Reductases - genetics warfarin westphalia |
title | Distribution of anticoagulant rodenticide resistance in Rattus norvegicus in the Netherlands according to Vkorc1 mutations |
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