Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

• Published in: BMC microbiology 2014-02, Vol.14 (1), p.46-46, Article 46
• Main Authors: Carrión, Víctor J, van der Voort, Menno, Arrebola, Eva, Gutiérrez-Barranquero, José A, de Vicente, Antonio, Raaijmakers, Jos M, Cazorla, Francisco M
• Format: Article
• Language: English
• Subjects: 2-component system; Analysis; artemis comparison tool; Artificial Gene Fusion; Bacterial Toxins - biosynthesis; Bacteriology; Biosynthesis; Cloning; Colleges & universities; E coli; escherichia-coli; Experiments; fluorescens pf-5; gaca; Gene Deletion; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Reporter; Genetic aspects; Genomics; Ligases; Lycopersicon esculentum; Mangifera indica; Peptide Synthases - metabolism; Physiological aspects; Plant Leaves - microbiology; Pseudomonas syringae; Pseudomonas syringae - genetics; Pseudomonas syringae - metabolism; Real-Time Polymerase Chain Reaction; response regulator; rna recognition; Signal transduction; signal-transduction pathway; Solanum lycopersicum - microbiology; tomato dc3000; virulence
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/1471-2180-14-46

The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis.