Marker-based estimation of heritability in immortal populations

• Published in: Genetics (Austin) 2015-02, Vol.199 (2), p.379-398
• Main Authors: Kruijer, Willem, Boer, Martin P, Malosetti, Marcos, Flood, Pádraic J, Engel, Bas, Kooke, Rik, Keurentjes, Joost J B, van Eeuwijk, Fred A
• Format: Article
• Language: English
• Subjects: Algorithms; Arabidopsis - genetics; arabidopsis-thaliana; Breeding; complex traits; Computational Biology - methods; Computer Simulation; Estimates; Flowers & plants; Genetic Markers; Genetics, Population; genome-wide association; Genome-Wide Association Study; Genomics; Genomics - methods; Genotype; Inheritance Patterns; Investigations; linear mixed models; missing heritability; Models, Genetic; multi-environment trials; Phenotype; plant-breeding trials; prediction; Quantitative genetics; quantitative trait loci; Quantitative Trait, Heritable; Reproducibility of Results; ROC Curve; selection; Software
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1534/genetics.114.167916

Heritability is a central parameter in quantitative genetics, from both an evolutionary and a breeding perspective. For plant traits heritability is traditionally estimated by comparing within- and between-genotype variability. This approach estimates broad-sense heritability and does not account for different genetic relatedness. With the availability of high-density markers there is growing interest in marker-based estimates of narrow-sense heritability, using mixed models in which genetic relatedness is estimated from genetic markers. Such estimates have received much attention in human genetics but are rarely reported for plant traits. A major obstacle is that current methodology and software assume a single phenotypic value per genotype, hence requiring genotypic means. An alternative that we propose here is to use mixed models at the individual plant or plot level. Using statistical arguments, simulations, and real data we investigate the feasibility of both approaches and how these affect genomic prediction with the best linear unbiased predictor and genome-wide association studies. Heritability estimates obtained from genotypic means had very large standard errors and were sometimes biologically unrealistic. Mixed models at the individual plant or plot level produced more realistic estimates, and for simulated traits standard errors were up to 13 times smaller. Genomic prediction was also improved by using these mixed models, with up to a 49% increase in accuracy. For genome-wide association studies on simulated traits, the use of individual plant data gave almost no increase in power. The new methodology is applicable to any complex trait where multiple replicates of individual genotypes can be scored. This includes important agronomic crops, as well as bacteria and fungi.