The chromosome copy number of the hyperthermophilic archaeon Thermococcus kodakarensis KOD1

The euryarchaeon Thermococcus kodakarensis is a well-characterized anaerobic hyperthermophilic heterotroph and due to the availability of genetic engineering systems it has become one of the model organisms for studying Archaea. Despite this prominent role among the Euryarchaeota, no data about the...

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Bibliographic Details
Published in:Extremophiles : life under extreme conditions 2015-07, Vol.19 (4), p.741-750
Main Authors: Spaans, Sebastiaan K., van der Oost, John, Kengen, Servé W. M.
Format: Article
Language:English
Subjects:
Archaea
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chromosomes
Chromosomes, Archaeal - genetics
Chromosomes, Archaeal - metabolism
Eukaryotes
Euryarchaeota
Genetic engineering
Genomes
Laboratorium voor Microbiologie
Life Sciences
Microbial Ecology
Microbiological Laboratory
Microbiologie
Microbiology
Microorganisms
Original Paper
Polyploidy
Prokaryotes
Pyrococcus
Space life sciences
Thermococcales
Thermococcus
Thermococcus - genetics
Thermococcus - metabolism
VLAG
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Summary:The euryarchaeon Thermococcus kodakarensis is a well-characterized anaerobic hyperthermophilic heterotroph and due to the availability of genetic engineering systems it has become one of the model organisms for studying Archaea. Despite this prominent role among the Euryarchaeota, no data about the ploidy level of this species is available. While polyploidy has been shown to exist in various Euryarchaeota, especially Halobacteria , the chromosome copy number of species belonging to one of the major orders within that phylum, i.e., the Thermococcales (including Thermococcus spp. and Pyrococcus spp.), has never been determined. This prompted us to investigate the chromosome copy number of T. kodakarensis . In this study, we demonstrate that T. kodakarensis is polyploid with a chromosome copy number that varies between 7 and 19 copies, depending on the growth phase. An apparent correlation between the presence of histones and polyploidy in Archaea is observed.
ISSN:1431-0651
1433-4909
DOI:10.1007/s00792-015-0750-5