QTL mapping of thrips resistance in pepper

• Published in: Theoretical and applied genetics 2015-10, Vol.128 (10), p.1945-1956
• Main Authors: Maharijaya, Awang, Vosman, Ben, Steenhuis-Broers, Greet, Pelgrom, Koen, Purwito, Agus, Visser, Richard G. F., Voorrips, Roeland E.
• Format: Article
• Language: English
• Subjects: Agriculture; Analysis; Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Capsicum - genetics; Capsicum annuum; Chromosome Mapping; Chromosomes, Plant; Crosses, Genetic; Diseases and pests; Females; Frankliniella occidentalis; Genetic aspects; Genetic Markers; Health aspects; Insect-plant relationships; Laboratorium voor Plantenveredeling; Laboratory of Plant Breeding; Larva; Leaves; Life Sciences; Observations; Original; Original Article; PBR Kwantitatieve aspecten; PBR Non host and insect resistance; PBR Non host en Insectenresistentie; PBR Quantitative aspects of Plant Breeding; Peppers; Plant Biochemistry; Plant Breeding; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant immunology; Plant resistance; Prevention; Quantitative Trait Loci; Thrips; Thysanoptera; Trichomes; Viruses; WUR PB Kwantitatieve Aspecten; WUR PB Non host en Insectenresistentie
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-015-2558-1

KEY MESSAGE : A QTL for thrips resistance on pepper chromosome 6 was identified and validated. This QTL affects thrips larval development and explains 50 % of the variation. Thrips is one of the most damaging pests in pepper (Capsicum). Resistance to thrips was identified in Capsicum annuum. This study was aimed at the elucidation of the genetic background of thrips resistance in Capsicum through QTL mapping. The QTL analysis was carried out for Frankliniella occidentalis resistance in an F₂ population consisting of 196 plants derived from an interspecific cross between the highly resistant C. annuum AC 1979 as female parent and the highly susceptible C. chinense 4661 as male parent. Fifty-seven SSR, 109 AFLP, and 5 SNP markers were used to construct a genetic map with a total length of 1636 cM. Damage caused by larvae and the survival of first and second instar larval stages observed in a no-choice test were used as parameters of resistance. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. Use of this marker as co-factor in a multiple-QTL mapping analysis failed to uncover any additional QTLs. This QTL explained about 50 % of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. Thrips resistance was not linked to trichome density.