The Double-Reduction Landscape in Tetraploid Potato as Revealed by a High-Density Linkage Map

The creation of genetic linkage maps in polyploid species has been a long-standing problem for which various approaches have been proposed. In the case of autopolyploids, a commonly used simplification is that random bivalents form during meiosis. This leads to relatively straightforward estimation...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 2015-11, Vol.201 (3), p.853-863
Main Authors: Bourke, Peter M, Voorrips, Roeland E, Visser, Richard G F, Maliepaard, Chris
Format: Article
Language:English
Subjects:
Centromere
Chromosome Mapping
Chromosome Pairing
Chromosomes
Computer Simulation
Double reduction
Genetic linkage
Genetic Markers
Genomics
Investigations
Linkage mapping
Multivalents
Potato
Potatoes
Recombination, Genetic
Solanum tuberosum
Solanum tuberosum - cytology
Solanum tuberosum - genetics
Tetraploid
Tetraploidy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The creation of genetic linkage maps in polyploid species has been a long-standing problem for which various approaches have been proposed. In the case of autopolyploids, a commonly used simplification is that random bivalents form during meiosis. This leads to relatively straightforward estimation of recombination frequencies using maximum likelihood, from which a genetic map can be derived. However, autopolyploids such as tetraploid potato (Solanum tuberosum L.) may exhibit additional features, such as double reduction, not normally encountered in diploid or allopolyploid species. In this study, we produced a high-density linkage map of tetraploid potato and used it to identify regions of double reduction in a biparental mapping population. The frequency of multivalents required to produce this degree of double reduction was determined through simulation. We also determined the effect that multivalents or preferential pairing between homologous chromosomes has on linkage mapping. Low levels of multivalents or preferential pairing do not adversely affect map construction when highly informative marker types and phases are used. We reveal the double-reduction landscape in tetraploid potato, clearly showing that this phenomenon increases with distance from the centromeres.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.115.181008