Novel TaqMan PCR screening methods for element cry3A and construct gat/T-pinII to support detection of both known and unknown GMOs

The import and use of genetically modified organisms (GMOs) is strictly regulated in the European Union. In order to maintain the legislation on GMOs, a genetic element screening is generally applied as a first step to detect authorised as well as unauthorised GMOs. Subsequent identification of GMOs...

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Bibliographic Details
Published in:European food research & technology 2017-03, Vol.243 (3), p.481-488
Main Authors: Prins, Theo W., van Hoof, Richard A., Scholtens, Ingrid M. J., Kok, Esther J.
Format: Article
Language:English
Subjects:
Agriculture
Analysis
Analytical Chemistry
Biotechnology
Chemistry
Chemistry and Materials Science
Corn
Detection
Food
Food Science
Forestry
Genes
Genetically altered foods
Genetically modified organisms
Identification
Identification systems
Laboratories
Legislation
Methods
Original Paper
PCR
qPCR
Real-time
Reference materials
Regulation
Screening
Soybeans
Studies
Toxins
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Summary:The import and use of genetically modified organisms (GMOs) is strictly regulated in the European Union. In order to maintain the legislation on GMOs, a genetic element screening is generally applied as a first step to detect authorised as well as unauthorised GMOs. Subsequent identification of GMOs that relate to the detected elements is performed by the application of event-specific detection methods. However, as the diversity of GMOs on the world market is increasing, there is an ongoing need for methods for additional informative screening elements. Genes that are increasingly applied in GMOs are cry3A (including variants mcry3A and eCry3.1Ab ) conferring resistance to Bt toxins, and gat , detoxifying glyphosate. Novel TaqMan PCR detection methods for element cry3A and construct gat /T- pinII were developed to support the identification of maize MIR604, 98140, 5307, canola 61061 and 73496, and soybean 356043. Also, other unknown (unauthorised) GMOs containing cry3A and/or gat /T- pinII can potentially be detected. Specificity, efficiency and sensitivity of the methods were evaluated.
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-016-2761-6