Photosynthetic induction and its diffusional, carboxylation and electron transport processes as affected by CO₂ partial pressure, temperature, air humidity and blue irradiance

• Published in: Annals of botany 2017-01, Vol.119 (1), p.191-205
• Main Authors: Kaiser, Elias, Kromdijk, Johannes, Harbinson, Jeremy, Heuvelink, Ep, Marcelis, Leo F. M.
• Format: Article
• Language: English
• Subjects: Carbon Dioxide - metabolism; Chlorophyll - metabolism; Dynamic photosynthesis, CO2 concentration, temperature, humidity, stomatal conductance, diffusional; Electron Transport - physiology; Humidity; Light; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - physiology; Original; ORIGINAL ARTICLES; Partial Pressure; Photosynthesis - physiology; Ribulose-Bisphosphate Carboxylase - metabolism; Temperature
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mcw226

Plants depend on photosynthesis for growth. In nature, factors such as temperature, humidity, CO partial pressure, and spectrum and intensity of irradiance often fluctuate. Whereas irradiance intensity is most influential and has been studied in detail, understanding of interactions with other factors is lacking. We tested how photosynthetic induction after dark-light transitions was affected by CO partial pressure (20, 40, 80 Pa), leaf temperatures (15·5, 22·8, 30·5 °C), leaf-to-air vapour pressure deficits (VPD ; 0·5, 0·8, 1·6, 2·3 kPa) and blue irradiance (0-20 %) in tomato leaves (Solanum lycopersicum). Rates of photosynthetic induction strongly increased with CO partial pressure, due to increased apparent Rubisco activation rates and reduced diffusional limitations. High leaf temperature produced slightly higher induction rates, and increased intrinsic water use efficiency and diffusional limitation. High VPD slowed down induction rates and apparent Rubisco activation and (at 2·3 kPa) induced damped stomatal oscillations. Blue irradiance had no effect. Slower apparent Rubisco activation in elevated VPD may be explained by low leaf internal CO partial pressure at the beginning of induction. The environmental factors CO partial pressure, temperature and VPD had significant impacts on rates of photosynthetic induction, as well as on underlying diffusional, carboxylation and electron transport processes. Furthermore, maximizing Rubisco activation rates would increase photosynthesis by at most 6-8 % in ambient CO partial pressure (across temperatures and humidities), while maximizing rates of stomatal opening would increase photosynthesis by at most 1-3 %.