Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

Multiplexed genome editing is simplified by harnessing the ability of Cpf1 to process its own pre-crRNA. Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be...

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Bibliographic Details
Published in:Nature biotechnology 2017-01, Vol.35 (1), p.31-34
Main Authors: Zetsche, Bernd, Heidenreich, Matthias, Mohanraju, Prarthana, Fedorova, Iana, Kneppers, Jeroen, DeGennaro, Ellen M, Winblad, Nerges, Choudhury, Sourav R, Abudayyeh, Omar O, Gootenberg, Jonathan S, Wu, Wen Y, Scott, David A, Severinov, Konstantin, van der Oost, John, Zhang, Feng
Format: Article
Language:English
Subjects:
631/1647/1513/1967
631/337/149
631/45/607/1160
Agriculture
Animals
Arrays
Bacterial Proteins - genetics
Bioinformatics
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
brief-communication
CRISPR
CRISPR-Associated Proteins - genetics
CRISPR-Cas Systems - genetics
Gene Editing - methods
Gene loci
Genomes
Laboratorium voor Microbiologie
Life Sciences
Methods
Mice
Microbiological Laboratory
Microbiologie
Microbiology
Observations
Projectmedewerkers Education & Research/QSI
Quantitative trait loci
RNA sequencing
RNA, Bacterial - genetics
Sequence Analysis, RNA - methods
VLAG
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Summary:Multiplexed genome editing is simplified by harnessing the ability of Cpf1 to process its own pre-crRNA. Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt.3737