Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats

• Published in: Research in veterinary science 2017-12, Vol.115, p.278-287
• Main Authors: Souriau, Armel, Freret, Sandrine, Foret, Benjamin, Willemsen, Peter T.J., Bakker, Douwe, Guilloteau, Laurence A.
• Format: Article
• Language: English
• Subjects: Animals; Antigen; Antigens; Antigens, Bacterial - isolation & purification; Cattle; Diagnosis; Early Diagnosis; Food contamination & poisoning; Gene expression; Goat; Goat Diseases - diagnosis; Goat Diseases - microbiology; Goats; IFN-γ; Immune response; Immune system; Immunology; Infections; Interferon; Interferon-gamma - metabolism; Interferon-gamma Release Tests - veterinary; Johne's disease; Life Sciences; Lipids; Lipids - pharmacology; Milk; Mycobacterium avium; Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis - immunology; Mycobacterium avium subsp. paratuberculosis - isolation & purification; Other; Paratuberculosis; Paratuberculosis - diagnosis; Paratuberculosis - microbiology; Pasteurization; Proteins; Recombinant Proteins - pharmacology; Tuberculin; Tuberculin - pharmacology; Tuberculosis; Veterinary medicine; γ-Interferon
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2017.05.025

Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis. Ten recombinant proteins were selected as potential candidates for the detection of MAP infection in young goats. They were found to detect 25 to 75% of infected shedder (IS) and infected non-shedder (INS) kids younger than 10months of age. In comparison, PPD was shown to detect only 10% of INS and no IS kids. For seven antigens, Se (21–33%) and Sp (≥90%) of IGRA were shown to be comparable with PPD at 20months old. Only three antigens were suitable candidates to detect IS adult goats, although Se was lower than that obtained with PPD. In paratuberculosis-free herds, IGRA results were negative in 97% of indoor goats and 86% of outdoor goats using the 10 antigens. However, 22 to 44% of one-year-old outdoor goats were positive suggesting that they may be infected. In conclusion, this study showed that ten MAP recombinant proteins are potential candidates for early detection of MAP infected goats. Combining these antigens could form a possible set of MAP antigens to optimize the Se of caprine IGRA. •The potential of 16 MAP recombinant proteins and five lipids was investigated in IFN-g release assays (IGRA) in MAP infected and non-infected goats.•Se of IGRA using ten MAP proteins was much higher than PPD to detect infected kids younger than 10 months of age.•Ten MAP proteins are potential candidates for early detection of MAP infected goats.