Response of a Predatory ant to Volatiles Emitted by Aphid- and Caterpillar-Infested Cucumber and Potato Plants

• Published in: Journal of chemical ecology 2017-10, Vol.43 (10), p.1007-1022
• Main Authors: Schettino, Mauro, Grasso, Donato A., Weldegergis, Berhane T., Castracani, Cristina, Mori, Alessandra, Dicke, Marcel, Van Lenteren, Joop C., Van Loon, Joop J. A.
• Format: Article
• Language: English
• Subjects: Agricultural ecosystems; Agriculture; Allelochemicals; Animals; Ant-plant interactions; Ants; Ants - physiology; Aphidoidea; Aphids - physiology; Aphis gossypii; Behavioural response; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Butterflies & moths; Caterpillars; Chemical ecology; Cucumbers; Cucumis sativus - physiology; Cues; Defense mechanisms; Ecology; Entomology; Food plants; Food sources; GC-MS analysis; Headspace; Herbivore-induced plant volatiles; Herbivores; Herbivory; Indirect defence; Infochemicals; Insects; Leaves; Lepidoptera - physiology; Life Sciences; Mixtures; Multivariate analysis; Odorants - analysis; Pest control; Plant defence; Plants; Potatoes; Predatory Behavior; Qualitative analysis; Solanum tuberosum - physiology; Tritrophic interactions; Vegetables; Volatile Organic Compounds - analysis; Volatile Organic Compounds - metabolism; Volatiles
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/s10886-017-0887-z

In response to herbivory by insects, various plants produce volatiles that attract enemies of the herbivores. Although ants are important components of natural and agro-ecosystems, the importance of herbivore-induced plant volatiles (HIPVs) as cues for ants for finding food sources have received little attention. We investigated responses of the ant Formica pratensis to volatiles emitted by uninfested and insect-infested cucumber ( Cucumis sativus ) and potato ( Solanum tuberosum ) plants. Cucumber plants were infested by the phloem-feeding aphid Aphis gossypii , the leaf chewer Mamestra brassicae or simultaneously by both insects. Potato plants were infested by either Aphis gossypii , by the leaf chewer Chrysodeixis chalcites or both. In olfactometer experiments, ants preferred volatile blends emitted by cucumber plants infested with M. brassicae caterpillars alone or combined with A. gossypii to volatiles of undamaged plants or plants damaged by A. gossypii only. No preference was recorded in choice tests between volatiles released by aphid-infested plants over undamaged plants. Volatiles emitted by potato plants infested by either C. chalcites or A. gossypii were preferred by ants over volatiles released by undamaged plants. Ants did not discriminate between potato plants infested with aphids and caterpillars over plants infested with aphids only. Plant headspace composition showed qualitative and/or quantitative differences between herbivore treatments. Multivariate analysis revealed clear separation between uninfested and infested plants and among herbivore treatments. The importance of HIPVs in indirect plant defence by ants is discussed in the context of the ecology of ant-plant interactions and possible roles of ants in pest management.