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Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors

Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and P...

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Bibliographic Details
Published in:Journal of applied phycology 2019-04, Vol.31 (2), p.981-997
Main Authors: Suzuki, Hirono, Hulatt, Chris J., Wijffels, René H., Kiron, Viswanath
Format: Article
Language:English
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Summary:Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L −1  day −1 and maximum cell density of 11.68 g L −1 were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g −1 dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g −1 dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g −1 dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L −1 ) while maintaining the protein, total PUFA, and omega-3 fatty acid contents.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-018-1606-z