Loading…
Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors
Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and P...
Saved in:
Published in: | Journal of applied phycology 2019-04, Vol.31 (2), p.981-997 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893 |
---|---|
cites | cdi_FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893 |
container_end_page | 997 |
container_issue | 2 |
container_start_page | 981 |
container_title | Journal of applied phycology |
container_volume | 31 |
creator | Suzuki, Hirono Hulatt, Chris J. Wijffels, René H. Kiron, Viswanath |
description | Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga
Koliella antarctica
(Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L
−1
day
−1
and maximum cell density of 11.68 g L
−1
were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g
−1
dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g
−1
dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g
−1
dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L
−1
) while maintaining the protein, total PUFA, and omega-3 fatty acid contents. |
doi_str_mv | 10.1007/s10811-018-1606-z |
format | article |
fullrecord | <record><control><sourceid>proquest_wagen</sourceid><recordid>TN_cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_541500</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2093012831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893</originalsourceid><addsrcrecordid>eNp1kU9v1DAQxS0EEkvhA3CzxNkwk6z_hFu1ogWxEhzo2ZrYTtdVGgc70Yp--noVJE5cZi7v9_RmHmPvET4igP5UEAyiADQCFSjx9ILtUOpWSNTqJdtB16AwncbX7E0pDwDQGTQ75m5zOi8nTpPnx4P4eXdzzeec_OqWmCaeBr6cAndp9II8zUvw_DG6nGi8J_49jTGMI1V6oVwJRzxOfD6lJfUx5UBuSbm8Za8GGkt493dfsbubL78OX8Xxx-23w_VRuD3CIppeSheC6smj9k420MDgQKowKE3odEN6aIOR5FzfA_X7VjmHRjuvlDdde8U-b75nug9TnOqwU80Vi00U7Rj7TPmPPa_ZTuNlzWtfrNyjBKjwhw2u1_9eQ1nsQ1rzVPPaBroWsDEtVhVuqvqCUnIY7Jzj48UVwV6KsFsRthZhL0XYp8o0G1OqtobK_5z_Dz0D-6OOtg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2093012831</pqid></control><display><type>article</type><title>Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Suzuki, Hirono ; Hulatt, Chris J. ; Wijffels, René H. ; Kiron, Viswanath</creator><creatorcontrib>Suzuki, Hirono ; Hulatt, Chris J. ; Wijffels, René H. ; Kiron, Viswanath</creatorcontrib><description>Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga
Koliella antarctica
(Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L
−1
day
−1
and maximum cell density of 11.68 g L
−1
were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g
−1
dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g
−1
dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g
−1
dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L
−1
) while maintaining the protein, total PUFA, and omega-3 fatty acid contents.</description><identifier>ISSN: 0921-8971</identifier><identifier>EISSN: 1573-5176</identifier><identifier>DOI: 10.1007/s10811-018-1606-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Arachidonic acid ; Biomedical and Life Sciences ; Cell culture ; Cell density ; Cold-adapted microalgae ; Dry weight ; Ecology ; Eicosapentaenoic acid ; Eicosapentaenoic acid (EPA) ; Fatty acids ; Flat-plate photobioreactor ; Freshwater & Marine Ecology ; Growth ; LC-PUFA ; Life Sciences ; Light intensity ; Lipids ; Luminous intensity ; Microalgae ; Nitrogen ; Oleic acid ; Omega-3 fatty acid ; Phosphate starvation response ; Phosphorus ; Photobioreactors ; Plant Physiology ; Plant Sciences ; Polyunsaturated fatty acids ; Proteins ; Starvation ; Stationary phase ; Triacylglycerol ; Triglycerides ; Weight</subject><ispartof>Journal of applied phycology, 2019-04, Vol.31 (2), p.981-997</ispartof><rights>The Author(s) 2018. corrected publication 2018</rights><rights>Journal of Applied Phycology is a copyright of Springer, (2018). All Rights Reserved. © 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893</citedby><cites>FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893</cites><orcidid>0000-0002-2741-8402</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Suzuki, Hirono</creatorcontrib><creatorcontrib>Hulatt, Chris J.</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Kiron, Viswanath</creatorcontrib><title>Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors</title><title>Journal of applied phycology</title><addtitle>J Appl Phycol</addtitle><description>Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga
Koliella antarctica
(Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L
−1
day
−1
and maximum cell density of 11.68 g L
−1
were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g
−1
dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g
−1
dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g
−1
dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L
−1
) while maintaining the protein, total PUFA, and omega-3 fatty acid contents.</description><subject>Arachidonic acid</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cold-adapted microalgae</subject><subject>Dry weight</subject><subject>Ecology</subject><subject>Eicosapentaenoic acid</subject><subject>Eicosapentaenoic acid (EPA)</subject><subject>Fatty acids</subject><subject>Flat-plate photobioreactor</subject><subject>Freshwater & Marine Ecology</subject><subject>Growth</subject><subject>LC-PUFA</subject><subject>Life Sciences</subject><subject>Light intensity</subject><subject>Lipids</subject><subject>Luminous intensity</subject><subject>Microalgae</subject><subject>Nitrogen</subject><subject>Oleic acid</subject><subject>Omega-3 fatty acid</subject><subject>Phosphate starvation response</subject><subject>Phosphorus</subject><subject>Photobioreactors</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polyunsaturated fatty acids</subject><subject>Proteins</subject><subject>Starvation</subject><subject>Stationary phase</subject><subject>Triacylglycerol</subject><subject>Triglycerides</subject><subject>Weight</subject><issn>0921-8971</issn><issn>1573-5176</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU9v1DAQxS0EEkvhA3CzxNkwk6z_hFu1ogWxEhzo2ZrYTtdVGgc70Yp--noVJE5cZi7v9_RmHmPvET4igP5UEAyiADQCFSjx9ILtUOpWSNTqJdtB16AwncbX7E0pDwDQGTQ75m5zOi8nTpPnx4P4eXdzzeec_OqWmCaeBr6cAndp9II8zUvw_DG6nGi8J_49jTGMI1V6oVwJRzxOfD6lJfUx5UBuSbm8Za8GGkt493dfsbubL78OX8Xxx-23w_VRuD3CIppeSheC6smj9k420MDgQKowKE3odEN6aIOR5FzfA_X7VjmHRjuvlDdde8U-b75nug9TnOqwU80Vi00U7Rj7TPmPPa_ZTuNlzWtfrNyjBKjwhw2u1_9eQ1nsQ1rzVPPaBroWsDEtVhVuqvqCUnIY7Jzj48UVwV6KsFsRthZhL0XYp8o0G1OqtobK_5z_Dz0D-6OOtg</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Suzuki, Hirono</creator><creator>Hulatt, Chris J.</creator><creator>Wijffels, René H.</creator><creator>Kiron, Viswanath</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TN</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>QVL</scope><orcidid>https://orcid.org/0000-0002-2741-8402</orcidid></search><sort><creationdate>20190401</creationdate><title>Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors</title><author>Suzuki, Hirono ; Hulatt, Chris J. ; Wijffels, René H. ; Kiron, Viswanath</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Arachidonic acid</topic><topic>Biomedical and Life Sciences</topic><topic>Cell culture</topic><topic>Cell density</topic><topic>Cold-adapted microalgae</topic><topic>Dry weight</topic><topic>Ecology</topic><topic>Eicosapentaenoic acid</topic><topic>Eicosapentaenoic acid (EPA)</topic><topic>Fatty acids</topic><topic>Flat-plate photobioreactor</topic><topic>Freshwater & Marine Ecology</topic><topic>Growth</topic><topic>LC-PUFA</topic><topic>Life Sciences</topic><topic>Light intensity</topic><topic>Lipids</topic><topic>Luminous intensity</topic><topic>Microalgae</topic><topic>Nitrogen</topic><topic>Oleic acid</topic><topic>Omega-3 fatty acid</topic><topic>Phosphate starvation response</topic><topic>Phosphorus</topic><topic>Photobioreactors</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polyunsaturated fatty acids</topic><topic>Proteins</topic><topic>Starvation</topic><topic>Stationary phase</topic><topic>Triacylglycerol</topic><topic>Triglycerides</topic><topic>Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Hirono</creatorcontrib><creatorcontrib>Hulatt, Chris J.</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Kiron, Viswanath</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oceanic Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>NARCIS:Publications</collection><jtitle>Journal of applied phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Hirono</au><au>Hulatt, Chris J.</au><au>Wijffels, René H.</au><au>Kiron, Viswanath</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors</atitle><jtitle>Journal of applied phycology</jtitle><stitle>J Appl Phycol</stitle><date>2019-04-01</date><risdate>2019</risdate><volume>31</volume><issue>2</issue><spage>981</spage><epage>997</epage><pages>981-997</pages><issn>0921-8971</issn><eissn>1573-5176</eissn><abstract>Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga
Koliella antarctica
(Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L
−1
day
−1
and maximum cell density of 11.68 g L
−1
were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g
−1
dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g
−1
dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g
−1
dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L
−1
) while maintaining the protein, total PUFA, and omega-3 fatty acid contents.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10811-018-1606-z</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-2741-8402</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0921-8971 |
ispartof | Journal of applied phycology, 2019-04, Vol.31 (2), p.981-997 |
issn | 0921-8971 1573-5176 |
language | eng |
recordid | cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_541500 |
source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
subjects | Arachidonic acid Biomedical and Life Sciences Cell culture Cell density Cold-adapted microalgae Dry weight Ecology Eicosapentaenoic acid Eicosapentaenoic acid (EPA) Fatty acids Flat-plate photobioreactor Freshwater & Marine Ecology Growth LC-PUFA Life Sciences Light intensity Lipids Luminous intensity Microalgae Nitrogen Oleic acid Omega-3 fatty acid Phosphate starvation response Phosphorus Photobioreactors Plant Physiology Plant Sciences Polyunsaturated fatty acids Proteins Starvation Stationary phase Triacylglycerol Triglycerides Weight |
title | Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T22%3A05%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wagen&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Growth%20and%20LC-PUFA%20production%20of%20the%20cold-adapted%20microalga%20Koliella%20antarctica%20in%20photobioreactors&rft.jtitle=Journal%20of%20applied%20phycology&rft.au=Suzuki,%20Hirono&rft.date=2019-04-01&rft.volume=31&rft.issue=2&rft.spage=981&rft.epage=997&rft.pages=981-997&rft.issn=0921-8971&rft.eissn=1573-5176&rft_id=info:doi/10.1007/s10811-018-1606-z&rft_dat=%3Cproquest_wagen%3E2093012831%3C/proquest_wagen%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c410t-2b55cee6bad17dc52020fc056ef67a1c72a7f3e85accbb0ab436cc187cd66d893%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2093012831&rft_id=info:pmid/&rfr_iscdi=true |