Tracking disease resistance deployment in potato breeding by enrichment sequencing

Summary Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) en...

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Bibliographic Details
Published in:Plant biotechnology journal 2019-02, Vol.17 (2), p.540-549
Main Authors: Armstrong, Miles R., Vossen, Jack, Lim, Tze Yin, Hutten, Ronald C. B., Xu, Jianfei, Strachan, Shona M., Harrower, Brian, Champouret, Nicolas, Gilroy, Eleanor M., Hein, Ingo
Format: Article
Language:English
Subjects:
Agricultural economics
Blight
breeding
Crop improvement
Crops
Crops, Agricultural
Diagnostic systems
Disease resistance
Disease Resistance - genetics
disease resistance genes
DNA sequencing
dRenSeq
Gene sequencing
Genes
Genomes
Genomics
Integrity
Late blight
Nematodes
Nucleotide sequencing
Phytophthora infestans - immunology
Plant Breeding
Plant Diseases - immunology
Plant Diseases - parasitology
Plant Proteins - genetics
Plants, Genetically Modified
Polymorphism, Genetic
potato
Potatoes
Progeny
Solanum tuberosum - genetics
Solanum tuberosum - immunology
Tetraploidy
Tracking
tracking of NLRs
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Summary:Summary Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high‐confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost‐effective in monitoring resistances than whole‐genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB‐LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.12997