Chromosomal instability in cell- and tissue cultures of tomato haploids and diploids

The effect of the tissue culture system, the genotype and the ploidy level of the plant material used as explant source on the stability of the ploidy level of plants regenerated from cell and tissue cultures of tomato was investigated. In addition the use of the chloroplast number in guard cells as...

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Bibliographic Details
Published in:Euphytica 1989, Vol.43 (1), p.179-186
Main Authors: Koornneef, M. (Landbouwuniversiteit Wageningen (Netherlands). Vakgroep Erfelijkheidsleer), Diepen, J.A.M. van, Hanhart, C.J, Kieboom-de Waart, A.C, Martinelli, L, Schoenmakers, H.C.H, Wijbrandi, J
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
AMELIORATION DES PLANTES
Biological and medical sciences
CELL CULTURE
CHROMOSOME NUMBER
CULTIVO DE CELULAS
CULTIVO DE TEJIDOS
CULTURE DE CELLULES
CULTURE DE TISSUS
Cytogenetics
DIPLOIDE
DIPLOIDS
Economic plant physiology
FITOMEJORAMIENTO
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
HAPLOIDE
HAPLOIDS
In vitro culture
Laboratorium voor Erfelijkheidsleer
Laboratory of Genetics
LYCOPERSICON
NOMBRE CHROMOSOMIQUE
NUMERO DE CROMOSOMAS
PLANT BREEDING
Pteridophyta, spermatophyta
TISSUE CULTURE
Vegetals and fungi
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Summary:The effect of the tissue culture system, the genotype and the ploidy level of the plant material used as explant source on the stability of the ploidy level of plants regenerated from cell and tissue cultures of tomato was investigated. In addition the use of the chloroplast number in guard cells as a measure for ploidy level was evaluated. Haploids of tomato were very instable, which instability was observed already in somatic root tip and leaf cells. The number of regenerated plants that retained the original ploidy level differed significantly between the tested haploids. The plants that were regenerated from leaf explants of diploids were predominantly diploid in contrast to the plants regenerated from established callus cultures and protoplast where the majority was tetraploid.
ISSN:0014-2336
1573-5060
DOI:10.1007/BF00037911