Dynamic Network Reconstruction from Gene Expression Data Describing the Effect of LiCl Stimulation on Hepatocytes

Wnt/β-catenin signalling plays an important role in zonation of liver parenchyma and in patterning of hepatocyte heterogeneity. A characteristic marker of this heterogeneity is glutamine synthetase, which is expressed only in a subset of pericentrally located hepatocytes. To investigate, whether and...

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Published in:Journal of integrative bioinformatics 2005-12, Vol.2 (1), p.58-73
Main Authors: Zellmer, Sebastian, Schmidt-Heck, Wolfgang, Gaunitz, Frank, Baldysiak-Figiel, Alicja, Guthke, Reinhardt, Gebhardt, Rolf
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Schmidt-Heck, Wolfgang
Gaunitz, Frank
Baldysiak-Figiel, Alicja
Guthke, Reinhardt
Gebhardt, Rolf
description Wnt/β-catenin signalling plays an important role in zonation of liver parenchyma and in patterning of hepatocyte heterogeneity. A characteristic marker of this heterogeneity is glutamine synthetase, which is expressed only in a subset of pericentrally located hepatocytes. To investigate, whether and how the Wnt/β-catenin signalling pathway is involved a culture of hepatocytes was stimulated by LiCl. This resulted in an increase in the specific GS activity, indicating that the Wnt/β-catenin pathway may participate in regulating GS levels. Affymetrix GeneChip oligonucleotide arrays were used to monitor the gene expression changes during a period from 2 to 24 hours after stimulation by LiCl. Samples from a cultivation without stimulation were used as controls. Based on the gene expression profiles a hypothetic signal transduction network was constructed by a reverse engineering algorithm. The network robustness was tested and the most stable structure was identified.
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