Study on The Method of Quantitative Analysis of Serum Ferritin and Soluble Transferrin Receptor with Protein Microarray Technology

• Published in: Biomedical and environmental sciences 2012-08, Vol.25 (4), p.430-439
• Main Authors: YIN, Ji Yong, SUN, Jing, HUANG, Jian, LI, Wen Xian, HUO, Jun Sheng
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - analysis; Combined measurement conditions; Ferritins - blood; Mice; Optimization; Protein Array Analysis - methods; Protein microarray; Rabbits; Receptors, Transferrin - blood; Serum ferritin; Soluble transferrin receptor; 单克隆抗体; 受体蛋白; 可溶性; 定量分析方法; 芯片技术; 蛋白质微阵列; 血清铁蛋白; 转铁蛋白受体
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/0895-3988.2012.04.008

Objective To establish and evaluate a protein serum ferritin （SF） and soluble transferrin receptor microarray method for combined measurement of （sTfR）. Methods Microarrayer was used to print both anti-SF antibodies I and anti-sTfR antibodies I on each protein microarray. Anti-SF antibodies II and anti-sTfR antibodies II were used as detection antibodies and goat antibodies coupled to Cy3 were used as antibodies Ill. The detection conditions of the quantitative analysis method for simultaneous measurement of SF and sTfR with protein microarray were optimized and evaluated. The protein microarray was compared with commercially available traditional tests with 26 serum samples. Results By comparison experiment, mouse monoclonal antibodies were chosen as the probes and contact printing was chosen as the printing method. The concentrations of SF and sTfR probes were 0.5 mg/mL and 0.5 mg/mL respectively, while those of SF and sTfR detection antibodies were 5 μg/mL and 0.36 μg/mL respectively. Intra- and inter-assay variability was between 3.26% and 18.38% for all tests. The regression coefficients comparing protein microarray with traditional test assays were better than 0.81 for SF and sTfR. Conclusion The present study has established a protein microarray method for combined measurement of SF and sTfR.